高效液相色谱结合柱后用乙酸锌进行光辐照法测定“纳豆”中的吡啶二羧酸
Determination of Dipicolinic Acid in "Natto" by High-Performance Liquid Chromatography Coupled With Postcolumn Photoirradiation With Zinc Acetate.
作者信息
Mawatari Ken-Ichi, Atsumi Motomasa, Nakamura Fumiya, Yasuda Makoto, Fukuuchi Tomoko, Yamaoka Noriko, Kaneko Kiyoko, Nakagomi Kazuya, Oku Naoto
机构信息
Department of Bioanalytical Chemistry, Faculty of Pharma-Science, Teikyo University, Tokyo, Japan.
出版信息
Int J Tryptophan Res. 2019 Jun 19;12:1178646919852120. doi: 10.1177/1178646919852120. eCollection 2019.
A system was developed for determining dipicolinic acid in "natto" using liquid chromatography with fluorometric detection. The compound was separated by reversed-phase chromatography using a mobile phase of 0.1 mol/L disodium hydrogen phosphate, 0.05 mol/L citric acid buffer (adjusted to pH 3.0) containing 3.0 mmol/L zinc acetate and 35 mmol/L perchloric acid. The compound in the column effluent was irradiated with ultraviolet light to produce fluorescence. This fluorescence was monitored at an excitation at 336 nm and an emission at 448 nm. The calibration curve for dipicolinic acid was observed to be linear in a range of 0.2 to 112 ng. The dipicolinic acid content of natto was 7.24 ± 0.54 mg/100 g (wet weight, mean ± standard deviation [SD], n = 6).
开发了一种使用液相色谱-荧光检测法测定“纳豆”中吡啶二羧酸的系统。该化合物通过反相色谱法分离，流动相为含有3.0 mmol/L乙酸锌和35 mmol/L高氯酸的0.1 mol/L磷酸氢二钠、0.05 mol/L柠檬酸缓冲液（调至pH 3.0）。柱流出物中的化合物用紫外光照射以产生荧光。在激发波长336 nm和发射波长448 nm处监测该荧光。观察到吡啶二羧酸的校准曲线在0.2至112 ng范围内呈线性。纳豆中吡啶二羧酸的含量为7.24±0.54 mg/100 g（湿重，平均值±标准差[SD]，n = 6）。
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