Akgun Feride Sinem, Sirin Duygu Yasar, Yilmaz Ibrahim, Karaarslan Numan, Ozbek Hanefi, Simsek Abdullah Talha, Kaya Yasin Emre, Kaplan Necati, Akyuva Yener, Caliskan Tezcan, Ates Ozkan
Department of Emergency Medicine, School of Medicine, Istanbul Maltepe University, Istanbul 34843, Turkey.
Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Namik Kemal University, Tekirdag 59100, Turkey.
Exp Ther Med. 2019 Jul;18(1):216-224. doi: 10.3892/etm.2019.7576. Epub 2019 May 13.
The present study aimed to evaluate the effects of dipyrone, an indispensable analgesic, anti-pyretic and anti-spasmodic used in emergency departments, on nucleus pulposus and annulus fibrosus cells . After surgical biopsy, primary cell cultures were prepared from intact intervertebral disc tissues. Dipyrone was administered to the cultures in the experimental groups except for the control group. The data obtained were statistically evaluated. The proliferation was identified to be suppressed via MTT analysis. The gene expression profile of the intervertebral disc cells in the dipyrone-treated groups was significantly changed. The expression of chondroadherin, cartilage oligo matrix protein, interleukin-1β and metalloproteinase (MMP)-19 genes were decreased, but MMP-13 and MMP-7 genes expressions were increased, as determined via reverse transcription-quantitative PCR. AO/PI staining revealed that no apoptotic or other type of cell death was detectable after administration of dipyrone does not mean that the drug is innocuous. The occurrence of cellular senescence and/or the halt of cell proliferation may also be important mechanisms underlying the adverse inhibitory effects of dipyrone. Therefore, prior to administering dipyrone in clinical practice, all possible adverse effects of this drug should be considered.
本研究旨在评估双氯芬酸（一种急诊科常用的不可或缺的镇痛、解热和解痉药物）对髓核和纤维环细胞的影响。手术活检后，从完整的椎间盘组织制备原代细胞培养物。除对照组外，对实验组的培养物给予双氯芬酸。对获得的数据进行统计学评估。通过MTT分析确定增殖受到抑制。双氯芬酸处理组中椎间盘细胞的基因表达谱发生了显著变化。通过逆转录定量PCR测定，软骨粘连蛋白、软骨寡聚基质蛋白、白细胞介素-1β和金属蛋白酶（MMP）-19基因的表达降低，但MMP-13和MMP-7基因的表达增加。AO/PI染色显示，给予双氯芬酸后未检测到凋亡或其他类型的细胞死亡，这并不意味着该药物无害。细胞衰老的发生和/或细胞增殖的停止也可能是双氯芬酸不良抑制作用的重要潜在机制。因此，在临床实践中使用双氯芬酸之前，应考虑该药物所有可能的不良反应。
