Bundscherer Anika C, Malsy Manuela, Gruber Michael A, Graf Bernhard M, Sinner Barbara
Department of Anesthesiology, University of Regensburg, Regensburg, Germany
Department of Anesthesiology, University of Regensburg, Regensburg, Germany.
Anticancer Res. 2018 Feb;38(2):745-751. doi: 10.21873/anticanres.12280.
BACKGROUND/AIM: The perioperative phase is supposed to be a period with high vulnerability for cancer dissemination. Acetaminophen and metamizole are common analgesics administered during this phase. We investigated the effect of acetaminophen, metamizole and 4-methylaminoantipyrine (MAA) on proliferation and apoptosis of colon carcinoma cell lines (SW 480 and HT 29).
Proliferation was detected by cell proliferation ELISA BrdU, and apoptosis by Annexin V staining. Cytochrome c and caspase 3, 8 and 9 expression levels were detected by western blot.
Acetaminophen, metamizole or MAA caused slight changes in proliferation. Acetaminophen, metamizole or the combination increased apoptosis in both cell lines. All agents decreased caspase 3 and 8 expression in SW480. Acetaminophen decreased caspase 9 expression in both cell lines.
In clinically relevant doses, acetaminophen and/or metamizole induce apoptosis in both colon cancer cell lines. Both mitochondrial and death receptor pathways might be involved in acetaminophen-induced apoptosis.
背景/目的：围手术期被认为是癌症播散高风险期。对乙酰氨基酚和安乃近是此阶段常用的镇痛药。我们研究了对乙酰氨基酚、安乃近和4-甲基氨基安替比林（MAA）对结肠癌细胞系（SW 480和HT 29）增殖和凋亡的影响。
通过细胞增殖ELISA BrdU检测增殖，通过膜联蛋白V染色检测凋亡。通过蛋白质免疫印迹法检测细胞色素c以及半胱天冬酶3、8和9的表达水平。
对乙酰氨基酚、安乃近或MAA对增殖有轻微影响。对乙酰氨基酚、安乃近或二者联用均可增加两种细胞系的凋亡。所有药物均降低SW480中半胱天冬酶3和8的表达。对乙酰氨基酚降低两种细胞系中半胱天冬酶9的表达。
在临床相关剂量下，对乙酰氨基酚和/或安乃近可诱导两种结肠癌细胞系凋亡。对乙酰氨基酚诱导的凋亡可能涉及线粒体和死亡受体途径。
