Ma Zhi, Lu Dezhi, Zhao Wanxin, Wang Huizhi
Department of Pediatric Surgery, Hongqi Hospital Affiliated to Mudanjiang Medical University, Mudanjiang, Heilongjiang 157000, P.R. China.
Department of Gynaecology and Obstetrics, Second Affiliated Hospital of Mudanjiang Medical University, Mudanjiang, Heilongjiang 157000, P.R. China.
Exp Ther Med. 2019 Jul;18(1):497-502. doi: 10.3892/etm.2019.7605. Epub 2019 May 23.
Expression levels of retinol-binding protein (RBP) and albumin (Alb) in the kidney of patients with neonatal hydronephrosis were detected to explore whether RBP and Alb can be used as diagnostic indicators for neonatal hydronephrosis. Blood and urine samples from neonates with hydronephrosis in the obstetrics and gynaecology department of Hongqi Hospital Affiliated to Mudanjiang Medical University from January 2016 to January 2018, and healthy newborns were collected. RBP and Alb levels in neonates, and the expression of IL-12 in serum of the mothers were detected by ELISA. To establish the rat model, 30 male Sprague Dawley (SD) rats were randomly divided into two groups: sham operation group (n=15) and unilateral ureteral obstruction (UUO) (n=15). Expression of RBP and Alb protein was detected by western blot analysis. Another 30 female SD rats were randomly divided into the Sham and IL-12 knockdown groups. The Sham group was given NC treatment, while IL-12 knockdown group was given treatment with IL-12 knockdown adenovirus through tail vein injection. Results of ELISA showed that serum RBP and renal urinary Alb levels were higher in neonates with hydronephrosis compared with the control group (P=0.009 or P=0.013). Compared with the control group, levels of IL-12 were significantly lower in mother of neonatal hydronephrosis group (P=0.001). After the IL-12 knockdown treatment in pregnant rats, levels of RBP and Alb hydronephrosis-related indicators were significantly increased. The incidence of hydronephrosis in rats delivered via IL-12 knockdown pregnant rats was significantly higher than that of normal pregnant rats (P=0.001). The combination of RBP and Alb in the diagnosis of neonatal hydronephrosis has important clinical significance, and neonatal hydronephrosis is closely related to the level of IL-12 in mothers before birth. Our findings provide new ideas and theoretical basis for the diagnosis and prediction of neonatal hydronephrosis.
检测新生儿肾积水患者肾脏中视黄醇结合蛋白（RBP）和白蛋白（Alb）的表达水平，以探讨RBP和Alb是否可作为新生儿肾积水的诊断指标。收集了2016年1月至2018年1月牡丹江医学院附属红旗医院妇产科新生儿肾积水患者及健康新生儿的血液和尿液样本。采用酶联免疫吸附测定法（ELISA）检测新生儿的RBP和Alb水平以及母亲血清中白细胞介素-12（IL-12）的表达。为建立大鼠模型，将30只雄性Sprague Dawley（SD）大鼠随机分为两组：假手术组（n = 15）和单侧输尿管梗阻（UUO）组（n = 15）。采用蛋白质免疫印迹法检测RBP和Alb蛋白的表达。另外30只雌性SD大鼠随机分为假手术组和IL-12基因敲除组。假手术组给予阴性对照处理，而IL-12基因敲除组通过尾静脉注射给予IL-12基因敲除腺病毒处理。ELISA结果显示，与对照组相比，肾积水新生儿血清RBP和肾脏尿Alb水平更高（P = 0.009或P = 0.013）。与对照组相比，新生儿肾积水组母亲的IL-12水平显著降低（P = 0.001）。对孕鼠进行IL-12基因敲除处理后，与肾积水相关的RBP和Alb指标水平显著升高。经IL-12基因敲除孕鼠分娩的大鼠肾积水发生率显著高于正常孕鼠（P = 0.001）。RBP和Alb联合诊断新生儿肾积水具有重要临床意义，且新生儿肾积水与母亲产前IL-12水平密切相关。我们的研究结果为新生儿肾积水的诊断和预测提供了新的思路和理论依据。
