通过光学成像对1型糖尿病小鼠皮肤疾病进行定量评估。
Quantitative evaluation of skin disorders in type 1 diabetic mice by optical imaging.
作者信息
Feng Wei, Zhang Chao, Yu Tingting, Zhu Dan
机构信息
Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology, Wuhan, Hubei 430074, China.
MoE Key Laboratory for Biomedical Photonics, Collaborative Innovation Center for Biomedical Engineering, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China.
出版信息
Biomed Opt Express. 2019 May 24;10(6):2996-3008. doi: 10.1364/BOE.10.002996. eCollection 2019 Jun 1.
Diabetes can affect the skin structure as well as the cutaneous vascular permeability. However, effective methods to quantitatively evaluate diabetes-induced skin disorders are still lacking. Here, we visualized the skin by using two-photon imaging and quantitatively evaluated the collagen morphology. The results indicated that diabetes could cause a significant reduction in the number of collagen fibers and lead to the disorder of skin collage fibers. And, the classic histological analysis also showed diabetes did lead to the change of skin filamentous structure. Additionally, the Evans Blue dye was used as an indicator to evaluate vascular permeability. We monitored cutaneous microvascular permeability by combining spectral imaging with the skin optical clearing method. This work is very useful for quantitative evaluation of skin disorders based on optical imaging, which has a great reference value in the clinical diagnosis.
糖尿病会影响皮肤结构以及皮肤血管通透性。然而，目前仍缺乏定量评估糖尿病引起的皮肤疾病的有效方法。在此，我们通过双光子成像对皮肤进行可视化，并定量评估胶原形态。结果表明，糖尿病会导致胶原纤维数量显著减少，并导致皮肤胶原纤维紊乱。而且，经典的组织学分析也表明糖尿病确实会导致皮肤丝状结构的改变。此外，伊文思蓝染料被用作评估血管通透性的指标。我们通过将光谱成像与皮肤光学透明方法相结合来监测皮肤微血管通透性。这项工作对于基于光学成像的皮肤疾病定量评估非常有用，在临床诊断中具有很大的参考价值。
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