Shi Rui, Guo Li, Zhang Chao, Feng Wei, Li Peng, Ding Zhihua, Zhu Dan
Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan, 430074, Hubei, P.R. China.
Department of Biomedical Engineering, Key Laboratory of Biomedical Photonics, Ministry of Education, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan, 430074, Hubei, P.R. China.
J Biophotonics. 2017 Jun;10(6-7):887-895. doi: 10.1002/jbio.201600221. Epub 2016 Dec 23.
Skin optical clearing has shown tremendous potential in improving various optical imaging performances, but there is some certain blindness in screening out high-efficiency in vivo optical clearing methods. In this work, three optical clearing agents: sucrose (Suc), fructose (Fruc) and PEG-400 (PEG), and two chemical penetration enhancers: propylene glycol (PG) and thiazone (Thiaz) were used. PEG was firstly mixed with the two penetration enhancers, respectively, and then mixed with Fruc and Suc, respectively, to obtain six kinds of skin optical clearing agents (SOCAs). Optical coherence tomography angiography was applied to monitor SOCAs-induced changes in imaging performances, skin optical properties, refractive index mismatching extent, and permeability rate. Experimental results demonstrated that PEG+Thiaz+Suc has the optimal capacity in enhancing the imaging performances, decreasing the scattering and the refractive index mismatching since Thiaz is superior to PG, and Suc is superior to Fruc. This study indicates that the optimal SOCA can be obtained directly by means of additionally adding or replacing the similar category substance in preexisting SOCAs with some more effective reagents. It not only provides an optimal SOCA, but also provides a useful way to develop more effective SOCAs. Cross-section skin structural texture (a), reconstructed blood flow distribution information (b), before or after treated with different SOCAs.
皮肤光学透明化在改善各种光学成像性能方面已显示出巨大潜力，但在筛选高效的体内光学透明化方法方面存在一定盲目性。在这项工作中，使用了三种光学透明剂：蔗糖（Suc）、果糖（Fruc）和聚乙二醇 - 400（PEG），以及两种化学渗透增强剂：丙二醇（PG）和噻唑（Thiaz）。首先将PEG分别与两种渗透增强剂混合，然后再分别与Fruc和Suc混合，得到六种皮肤光学透明剂（SOCAs）。应用光学相干断层扫描血管造影术来监测SOCAs引起的成像性能、皮肤光学特性、折射率失配程度和渗透率的变化。实验结果表明，由于噻唑优于丙二醇，蔗糖优于果糖，PEG + Thiaz + Suc在增强成像性能、降低散射和折射率失配方面具有最佳能力。这项研究表明，通过在现有SOCAs中额外添加或用更有效的试剂替代类似类别的物质，可以直接获得最佳的SOCAs。它不仅提供了一种最佳的SOCAs，还提供了一种开发更有效SOCAs的有用方法。不同SOCAs处理前后的横截面皮肤结构纹理（a）、重建的血流分布信息（b）。
