Department of Chemistry, Faculty of Engineering , Istanbul University-Cerrahpasa , Avcilar, 34320 Istanbul , Turkey.
Turkish Academy of Sciences (TUBA) , Piyade Street 27 , Cankaya, 06690 Ankara , Turkey.
J Agric Food Chem. 2019 Aug 21;67(33):9187-9202. doi: 10.1021/acs.jafc.9b03657. Epub 2019 Aug 6.
The rationale and scope of the main issues of antioxidant measurement are presented, with basic definitions and terms in antioxidant research (such as reactive species and related antioxidative defenses, oxidative stress, and antioxidant activity and capacity) in a historical background. An overview of technical problems and expectations is given in terms of interpretation of results, precision and comparability of methods, capability of simulating physical reality, and analytical performance (sensitivity, selectivity, etc.). Current analytical methods for measuring antioxidant and antiradical activity are classified from various viewpoints. Reaction kinetics and thermodynamics of current analytical methods are discussed, describing physicochemical aspects of antioxidant action and measurement. Controversies and limitations of the widely used antioxidant assays are elaborated in detail. Emerging techniques in antioxidant testing (e.g., nanotechnology, sensors, electrochemistry, chemometry, and hyphenated methods) are broadly introduced. Finally, hints for the selection of suitable assays (i.e., preferable for a specific purpose) and future prospects are given.
本文介绍了抗氧化剂测量的基本原理和范围，以及抗氧化研究中的基本定义和术语（如活性物质和相关抗氧化防御、氧化应激以及抗氧化活性和能力），并附有历史背景。本文还概述了技术问题和预期，包括结果解释、方法的精度和可比性、模拟物理现实的能力以及分析性能（灵敏度、选择性等）。目前用于测量抗氧化和抗自由基活性的分析方法从不同角度进行了分类。本文讨论了当前分析方法的反应动力学和热力学，描述了抗氧化作用和测量的物理化学方面。详细阐述了广泛使用的抗氧化剂测定方法的争议和局限性。本文还广泛介绍了抗氧化测试中的新兴技术（例如纳米技术、传感器、电化学、化学计量学和联用方法）。最后，给出了选择合适测定方法（即针对特定目的的优选方法）的提示和未来展望。
