Magalhães Luís M, Segundo Marcela A, Reis Salette, Lima José L F C
REQUIMTE, Serviço de Química-Física, Faculdade de Farmácia, Universidade do Porto, Rua Aníbal Cunha, 164, 4099-030 Porto, Portugal.
Anal Chim Acta. 2008 Apr 14;613(1):1-19. doi: 10.1016/j.aca.2008.02.047. Epub 2008 Feb 29.
Several of the most commonly used methods for in vitro determination of antioxidant capacity are reviewed in the present paper. The chemical principles of methods based either on biological oxidants (peroxyl radical, superoxide radical anion, hydrogen peroxide, hydroxyl radical, hypochlorous acid, singlet oxygen, nitric oxide radical, and peroxynitrite) or on non-biological assays (scavenging of 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulphonate) radical cation (TEAC assay), scavenging of 2,2-diphenyl-1-picrylhydrazyl radical (DPPH assay), ferric reducing antioxidant power (FRAP assay), Folin-Ciocalteu reducing capacity (FC assay), electrochemical total reducing capacity) are outlined and critically discussed. The scope of application, the advantages and shortcomings of each method are also highlighted.
本文综述了几种最常用的体外抗氧化能力测定方法。概述并批判性地讨论了基于生物氧化剂（过氧自由基、超氧阴离子自由基、过氧化氢、羟基自由基、次氯酸、单线态氧、一氧化氮自由基和过氧亚硝酸盐）或基于非生物测定法（2,2'-偶氮双（3-乙基苯并噻唑啉-6-磺酸盐）自由基阳离子清除法（TEAC法）、2,2-二苯基-1-苦基肼自由基清除法（DPPH法）、铁还原抗氧化能力（FRAP法）、福林-西奥尔特还原能力（FC法）、电化学总还原能力）的方法的化学原理。还强调了每种方法的应用范围、优点和缺点。
