Department of Biochemistry, School of Life Sciences, University of KwaZulu- Natal (Westville Campus), Durban 4000, Durban, South Africa.
School of Chemistry and Physics University of KwaZulu-Natal (Westville Campus), Durban 4000, Durban, South Africa.
Acta Pharm. 2018 Dec 1;68(4):425-439. doi: 10.2478/acph-2018-0037.
In this study, we identified bioactive compounds from the ethanolic extracts of the leaves, stem bark and root bark of Acalypha wilkesiana through GC-MS analysis and investigated the effects of these extracts on some of the enzymes linked to type 2 diabetes. Plant parts were extracted sequentially with ethyl acetate, ethanol and water. GC-MS analysis revealed the presence of long-chain alkyl acids, esters, ketones and alcohols including phytol and phytol acetate along with some secondary metabolites such as xanthone, vitamin E and various types of sterols including stigmasterol, campesterol and sitosterol. Ethanolic extracts of all the parts showed a dose- -dependent inhibition of α-glucosidase and α-amylase activity. The extracts also demonstrated anti-lipase activity. The ethanolic extract of root bark showed the highest inhibition of enzymes compared to other extracts. The EC50 values (concentrations for 50 % inhibition) of α-glucosidase, α-amylase and lipase inhibition were 35.75 ± 1.95, 6.25 ± 1.05 and 101.33 ± 5.21 μg mL-1, resp. The study suggests that A. wilkesiana ethanolic extracts have the ability to inhibit the activity of enzymes linked to type 2 diabetes. Further studies are needed to confirm the responsible bioactive compounds in this regard.
在这项研究中，我们通过 GC-MS 分析从 Acalypha wilkesiana 的叶、茎皮和根皮的乙醇提取物中鉴定出了生物活性化合物，并研究了这些提取物对与 2 型糖尿病相关的一些酶的影响。植物部分依次用乙酸乙酯、乙醇和水提取。GC-MS 分析显示存在长链烷基酸、酯、酮和醇，包括植醇和植醇乙酸酯，以及一些次生代谢物，如黄烷酮、维生素 E 和各种甾醇，包括豆甾醇、菜油甾醇和谷甾醇。所有部位的乙醇提取物均表现出对α-葡萄糖苷酶和α-淀粉酶活性的剂量依赖性抑制作用。提取物还表现出抗脂肪酶活性。与其他提取物相比，根皮的乙醇提取物对酶的抑制作用最强。α-葡萄糖苷酶、α-淀粉酶和脂肪酶抑制的 EC50 值（抑制 50%的浓度）分别为 35.75 ± 1.95、6.25 ± 1.05 和 101.33 ± 5.21 μg mL-1。研究表明，A. wilkesiana 乙醇提取物具有抑制与 2 型糖尿病相关的酶活性的能力。需要进一步的研究来确认这方面的有效生物活性化合物。
