Nishimura T, Uchiyama Y, Hashimoto Y
Department of Hygienic Chemistry, Tohoku University, Sendai, Japan.
Cell Immunol. 1988 Mar;112(1):220-5. doi: 10.1016/0008-8749(88)90290-0.
Culture of C57BL/6 mouse spleen cells with syngeneic EL4 lymphoma cells resulted in no induction of killer cells reactive against EL4 cells. However, in vitro sensitization of C57BL/6 mouse spleen cells with interleukin 2 (IL-2)-producing EL4 lymphoma cells caused the generation of lymphokine-activated killer (LAK) cells, which lyse a variety of tumor cells. Consistent with an in vitro system, we demonstrate that Thy 1.2+, Ly2+, asialo GM1+ LAK cells were successfully induced by in vivo immunization with syngeneic IL-2-producing EL4 lymphoma cells.
将C57BL/6小鼠脾细胞与同基因EL4淋巴瘤细胞共培养，未诱导出对EL4细胞有反应的杀伤细胞。然而，用产生白细胞介素2（IL-2）的EL4淋巴瘤细胞对C57BL/6小鼠脾细胞进行体外致敏，可导致淋巴因子激活的杀伤（LAK）细胞的产生，这些细胞可裂解多种肿瘤细胞。与体外系统一致，我们证明，用同基因产生IL-2的EL4淋巴瘤细胞进行体内免疫可成功诱导出Thy 1.2+、Ly2+、无唾液酸GM1+的LAK细胞。
