Skinner M, Marbrook J
Department of Immunobiology, School of Medicine, University of Auckland, New Zealand.
Cell Immunol. 1988 Mar;112(1):104-11. doi: 10.1016/0008-8749(88)90279-1.
Cells with cytolytic activity can be detected in mouse fetal thymic lobes cultured in the presence of interleukin 2 for 6 days. The lymphokine-activated killer cells from 14-day fetal thymic lobes are relatively resistant to treatment with anti-Ly-2 antibody and complement (CD8-) but sensitive to anti-Thy-1 and complement treatment (Thy-1+). They display major histocompatibility complex-unrestricted killing, lysing both syngeneic and allogeneic tumor cells, but will not lyse human xenogenic target cells. Low levels of cytotoxic activity can be detected in thymic lobes from Day 12-13 embryos and this activity increases with embryonic age. While the events which lead to the inhibition of normal maturation of fetal thymocytes by inclusion of IL-2 in fetal thymus organ cultures are unknown, the appearance of cytotoxic cells raises the question of whether they are involved in the normal intrathymic cell death process.
在含有白细胞介素2的条件下培养6天的小鼠胎儿胸腺叶中，可以检测到具有细胞溶解活性的细胞。来自14天胎儿胸腺叶的淋巴因子激活的杀伤细胞对抗Ly-2抗体和补体（CD8-）处理相对耐药，但对抗Thy-1和补体处理敏感（Thy-1+）。它们表现出主要组织相容性复合体非限制性杀伤作用，能溶解同基因和异基因肿瘤细胞，但不会溶解人异种靶细胞。在12至13天胚胎的胸腺叶中可检测到低水平的细胞毒性活性，且这种活性随胚胎年龄增加而增强。虽然在胎儿胸腺器官培养物中加入IL-2导致胎儿胸腺细胞正常成熟受到抑制的机制尚不清楚，但细胞毒性细胞的出现引发了一个问题，即它们是否参与正常的胸腺内细胞死亡过程。
