基于 NMR 的代谢组学方法研究铁棍山药中代谢产物的分布特征。

NMR-based metabolomics approach to investigate the distribution characteristics of metabolites in Dioscorea opposita Thunb. cv. Tiegun.

机构信息

Institute of Quality Standard and Testing Technology for Agro-products, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China; Key Laboratory of Grain Quality and Safety and Testing Henan Province, Zhengzhou 450002, China.

Department of Pharmacy, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.

出版信息

Food Chem. 2019 Nov 15;298:125063. doi: 10.1016/j.foodchem.2019.125063. Epub 2019 Jun 24.

DOI:10.1016/j.foodchem.2019.125063

PMID:31260979

Abstract

Dioscorea opposita Thunb. cv. Tiegun (DTT), a type of homologous medicinal plant, is commonly used as food in daily life. However, there has always been confusion regarding removal of the peel, as the nutrient metabolite composition of the peel is unclear. Here, a nuclear magnetic resonance (NMR)-based metabolomics approach was used to determine the metabolite distribution in DTT exclude-peel and peel. Thirteen characteristic metabolites with statistical significance were identified and compared using multivariate, univariate and cluster analyses. The results demonstrated that the peel contained the higher levels of α-glucose, batatasin IV, batatasin I, asparagine, β-glucose, protodioscin, threonine, protogracillin, dioscin, and β-sitosteryl acetate, and the samples without the peel had the higher levels of leucine, glutamine and alanine. This study provided scientific data for understanding the distribution characteristics of metabolites in DTT samples, promoting reasonable consumption of DTT.

摘要

盾叶薯蓣 cv. 铁根（DTT）是同源药用植物的一种，在日常生活中常被用作食物。然而，关于是否去皮一直存在争议，因为人们不清楚薯蓣皮的营养代谢物组成。本研究采用基于核磁共振（NMR）的代谢组学方法，测定了 DTT 去皮和带皮样品中的代谢物分布。通过多元、单变量和聚类分析，鉴定并比较了 13 种具有统计学意义的特征代谢物。结果表明，薯蓣皮中含有更高水平的α-葡萄糖、蝙蝠葛苏林 IV、蝙蝠葛苏林 I、天冬酰胺、β-葡萄糖、原薯蓣皂甙、苏氨酸、原纤细薯蓣皂甙、薯蓣皂甙、β-谷甾醇乙酸酯，而无皮样品中则含有更高水平的亮氨酸、谷氨酰胺和丙氨酸。本研究为了解 DTT 样品中代谢物的分布特征提供了科学数据，促进了 DTT 的合理消费。