Bartlett J M, Kerr J B, Sharpe R M
MRC Reproductive Biology Unit, Centre for Reproductive Biology, Edinburgh, Scotland, United Kingdom.
J Androl. 1988 Jan-Feb;9(1):31-40. doi: 10.1002/j.1939-4640.1988.tb01006.x.
Testicular weight and morphology, serum gonadotropins, intratesticular levels of testosterone and ABP levels in testicular interstitial fluid were studied in adult rats at intervals of 1 to 70 days after a single oral dose of 650 mg/kg methoxy acetic acid. At 3 days, this treatment resulted in the selective loss or depletion of pachytene and later spermatocytes from seminiferous tubules at all stages other than VIII to XI of the spermatogenic cycle. At later times this lesion was expressed as an absence mainly of round (14 days) or elongated (21 days) spermatids from the majority of seminiferous tubules. Other than these changes, spermatogenesis did not appear to be affected by treatment and was qualitatively normal in all tubules at 70 days after treatment. As deduced from cell counts at 3 days posttreatment, the initial action of methoxy acetic acid was restricted to late zygotene spermatocytes (stage XII) and pachytene spermatocytes at all stages other than early- to mid-stage VII. Levels of FSH in serum and those of ABP in testicular interstitial fluid indicated that Sertoli cell function was altered in rats treated with methoxy acetic acid. Both were increased at 1 to 3 days posttreatment, returned to normal at 7 to 14 days but were increased again at 21 days before finally returning to control levels at 28 days. In contrast, the levels of testosterone in serum, isolated seminiferous tubules and testicular interstitial fluid were unaffected by treatment, as also were the serum levels of LH. The two periods of increase in FSH and ABP levels coincided with the times of greatest decrease (approximately 20%) in testicular weight, and may be related either to the type of germ cell missing from the affected tubules and/or to the stage of the cycle of the affected (or unaffected) tubules. These data suggest that chemicals such as methoxy acetic acid may prove useful in the study of paracrine interactions in vivo.
在成年大鼠单次口服650mg/kg甲氧基乙酸后的1至70天内，每隔一段时间研究其睾丸重量和形态、血清促性腺激素、睾丸间质液中睾酮的睾丸内水平和ABP水平。在第3天，这种处理导致生精周期VIII至XI期以外所有阶段的曲细精管中粗线期及后期精母细胞选择性缺失或耗竭。在之后的时间里，这种损伤主要表现为大多数曲细精管中圆形（14天）或长形（21天）精子细胞的缺失。除了这些变化外，生精过程似乎未受处理影响，在处理后70天时所有曲细精管中的生精过程在质量上是正常的。从处理后第3天的细胞计数推断，甲氧基乙酸的初始作用仅限于晚偶线期精母细胞（XII期）以及VII期早期至中期以外所有阶段的粗线期精母细胞。血清中FSH水平和睾丸间质液中ABP水平表明，用甲氧基乙酸处理的大鼠中支持细胞功能发生了改变。二者在处理后1至3天升高，在7至14天恢复正常，但在21天再次升高，最终在28天恢复到对照水平。相比之下，血清、分离的曲细精管和睾丸间质液中的睾酮水平不受处理影响，血清LH水平也是如此。FSH和ABP水平升高的两个时期与睾丸重量最大降幅（约20%）的时间一致，可能与受影响曲细精管中缺失的生殖细胞类型和/或受影响（或未受影响）曲细精管的周期阶段有关。这些数据表明，诸如甲氧基乙酸之类的化学物质可能在体内旁分泌相互作用的研究中证明是有用的。
