Ashtari Simin, Khademvatan Shahram, Abasi Esmaeil, Aminpour Arash
2Department of Parasitology and Mycology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
1Cellular and Molecular Research Center, Urmia University of Medical Sciences, Urmia, Iran.
J Parasit Dis. 2019 Jun;43(2):270-275. doi: 10.1007/s12639-019-01088-5. Epub 2019 Jan 29.
Toxocariasis is a helminthic zoonosis caused by larval stages of the roundworm of dog, , and less frequently by , the roundworm of cats. Eosinophilia in peripheral blood may be indicative of a disease; however, it does not necessarily represent toxocariasis. Therefore, it is necessary to investigate the relationship between eosinophilia and toxocariasis in a region. The aims of this study were the diagnosis of hyperosinophilia patients using traditional ELISA kit and also by a handmade ELISA kit produced by excretory-secretory (TCES) antigens as well as the determination of the abundance of anti- antibodies among people referred to Urmia, northwest of Iran care centers. Traditional ELISA kit was used to determine anti-TCES-specific IgG antibodies on 180 hypereosinophilic samples. These antibodies were evaluated in 1002 samples, including 180 hypereosinophilic samples and 822 random samples without eosinophilia by a handmade ELISA kit produced by TCES antigens. A Western-blot confirmatory test was performed on ELISA-positive samples. Our results showed a 17.22% prevalence rate of antibodies among hypereosinophilic samples with traditional ELISA kit, and this rate was 3.89% in the 1002 study population with random sampling (with or without eosinophilia). Also, there was a good match between the results of handmade ELISA with those of traditional kit. The positive results in the ELISA method were confirmed by the Western-blot analysis. Our findings show that although the high eosinophil count is not necessarily a sign of toxocariasis, in Urmia distric, about one-fifth of eosinophilia cases have anti-toxocariasis antibodies. In addition, the abundance of anti-Toxocara antibodies in this area was 3.89%.
弓首蛔虫病是一种由犬弓首蛔虫的幼虫阶段引起的蠕虫性人畜共患病，较少由猫弓首蛔虫引起。外周血嗜酸性粒细胞增多可能提示疾病，但不一定代表弓首蛔虫病。因此，有必要在一个地区研究嗜酸性粒细胞增多与弓首蛔虫病之间的关系。本研究的目的是使用传统酶联免疫吸附测定（ELISA）试剂盒以及由排泄分泌（TCES）抗原制成的手工ELISA试剂盒诊断嗜酸性粒细胞增多症患者，并确定转诊至伊朗西北部乌尔米耶护理中心的人群中抗弓首蛔虫抗体的丰度。使用传统ELISA试剂盒测定180份嗜酸性粒细胞增多症样本中的抗TCES特异性IgG抗体。通过由TCES抗原制成的手工ELISA试剂盒在1002份样本中评估这些抗体，其中包括180份嗜酸性粒细胞增多症样本和822份无嗜酸性粒细胞增多的随机样本。对ELISA阳性样本进行蛋白质印迹确证试验。我们的结果显示，使用传统ELISA试剂盒时，嗜酸性粒细胞增多症样本中抗弓首蛔虫抗体的患病率为17.22%，在1002名随机抽样（有或无嗜酸性粒细胞增多）的研究人群中，这一患病率为3.89%。此外，手工ELISA的结果与传统试剂盒的结果匹配良好。ELISA方法的阳性结果通过蛋白质印迹分析得到证实。我们的研究结果表明，虽然高嗜酸性粒细胞计数不一定是弓首蛔虫病的标志，但在乌尔米耶地区，约五分之一的嗜酸性粒细胞增多症病例具有抗弓首蛔虫抗体。此外，该地区抗弓首蛔虫抗体的丰度为3.89%。
