Ortega-Peña Silvestre, Franco-Cendejas Rafael, Salazar-Sáenz Brenda, Rodríguez-Martínez Sandra, Cancino-Díaz Mario E, Cancino-Díaz Juan C
Laboratorio de Infectología, Instituto Nacional de Rehabilitación Luis Guillermo Ibarra Ibarra.
Departamento de Microbiología e Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional. Ciudad de México, México.
Cir Cir. 2019;87(4):428-435. doi: 10.24875/CIRU.19000690.
To determine the prevalence and virulence factors of coagulase-negative Staphylococci (CNS) in prosthetic joint infections (PJI).
CNS were isolated of 66 hip and knee PJI from Instituto Nacional de Rehabilitación Luis Guillermo Ibarra Ibarra, México City. Antimicrobial susceptibility and biofilm formation in CNS were determined; icaADBC, aap, bap and embp genes were determined by PCR.
Staphylococcus and Staphylococcus hominis were the most prevalent with 82 y 80% respectively. Staphylococcus lugdunensis, Staphylococcus haemolyticus, Staphylococcus capitis, Staphylococcus caprae, Staphylococcus sciuri and Staphylococcus lentus were less frequent. The majority of isolates were resistant to β-lactam antibiotics, fluoroquinolone, and erythromycin. 41% of CNS were biofilm former and 59% were non-biofilm former (p = 0.0551). Biofilm former Staphylococcus epidermidis showed a high presence of icaADBC, aap and embp operons compared to the non-biofilm former isolates (p < 0.05). In contrast, non-S. epidermidis CNS had only the aap gen.
S. haemolyticus, S. sciuri and S. lentus are new isolates of PJI not previously reported with virulence factors similar to CNS isolates.
确定凝固酶阴性葡萄球菌（CNS）在人工关节感染（PJI）中的患病率及毒力因子。
从墨西哥城路易斯·吉列尔莫·伊瓦拉·伊瓦拉国家康复研究所的66例髋膝关节PJI中分离出CNS。测定CNS的抗菌药敏性及生物膜形成情况；通过聚合酶链反应（PCR）检测icaADBC、aap、bap和embp基因。
表皮葡萄球菌和人葡萄球菌最为常见，分别占82%和80%。路邓葡萄球菌、溶血葡萄球菌、头葡萄球菌、山羊葡萄球菌、松鼠葡萄球菌和缓慢葡萄球菌较少见。大多数分离株对β-内酰胺类抗生素、氟喹诺酮类和红霉素耐药。41%的CNS为生物膜形成菌，59%为非生物膜形成菌（p = 0.0551）。与非生物膜形成菌分离株相比，生物膜形成菌表皮葡萄球菌的icaADBC、aap和embp操纵子高度存在（p < 0.05）。相比之下，非表皮葡萄球菌CNS仅具有aap基因。
溶血葡萄球菌、松鼠葡萄球菌和缓慢葡萄球菌是PJI的新分离株，此前未报道其具有与CNS分离株相似的毒力因子。
