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利用从美味侧耳中分离得到的单核菌株通过固态发酵法对水稻和向日葵副产物进行生物炼制。

Biorefining via solid-state fermentation of rice and sunflower by-products employing novel monosporic strains from Pleurotus sapidus.

机构信息

Laboratory of Biotechnology of Edible and Medicinal Mushrooms, Centro de Recursos Naturales Renovables de la Zona Semiárida (CERZOS-UNS/CONICET), Camino de La Carrindaga Km7, 8000 Bahía Blanca, Buenos Aires, Argentina.

Universidad de la República (UdelaR), Facultad de Ciencias, CIN, Mataojos s/n, Malvin Norte, Montevideo, 11200 Montevideo, Uruguay.

出版信息

Bioresour Technol. 2019 Oct;289:121692. doi: 10.1016/j.biortech.2019.121692. Epub 2019 Jun 22.

Abstract

Pleurotus sapidus monokaryotic strains (Mk) were screened as a novel source of mycelia to valorize rice straw (RS), rice husks (RH) and sunflower seed hulls (SSH) into value-added products through solid-state fermentation (SSF). P. sapidus Dk3174 basidiospores were cultured in the presence of Remazol Brillant Blue R for strain selection, revealing the ligninolytic ability of emerging colonies. Further screening demonstrated the intraspecific variability in dye degradation and enzyme production of 63 strains. Growth rate, biomass and enzyme production in plates containing RS, RH or SSH pointed at MkP6 as a suitable strain for pilot-scale SSF. MkP6 presented a similar laccase profile as the parental Dk3174, being greater in pasteurized substrates (300-1200 U/Kg) than in sterilized substrates (30-250 U/Kg). Peroxidase represented 25% of the total ligninolytic activity measured. The SSH fermented biomass with MkP6 obtained good yields of nanocellulose (67%) and the saccharide release for ethanol production increased by 3-4 times.

摘要

美味侧耳单核菌株(Mk)被筛选为一种新型的菌丝体来源,通过固态发酵(SSF)将稻草(RS)、稻壳(RH)和葵花籽壳(SSH)增值为附加值产品。在存在 Remazol Brillant Blue R 的情况下培养美味侧耳 Dk3174 担孢子,揭示了新兴菌落的木质素降解能力。进一步筛选表明,63 株菌株在染料降解和酶生产方面存在种内变异性。在含有 RS、RH 或 SSH 的平板上的生长速度、生物量和酶生产表明 MkP6 是适合进行中试 SSF 的合适菌株。MkP6 的漆酶谱与亲本 Dk3174 相似,在巴氏消毒底物(300-1200 U/Kg)中的比在灭菌底物(30-250 U/Kg)中的更大。过氧化物酶占测定的总木质素降解活性的 25%。用 MkP6 发酵 SSH 生物质获得了纳米纤维素(67%)的良好产量,乙醇生产的糖释放增加了 3-4 倍。

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