Xu Shuai, Wang Fei, Fu Yongping, Li Dan, Sun Xiaozhong, Li Changtian, Song Bing, Li Yu
Engineering Research Centre of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University Changchun 130118 P. R. China
Jilin Province Product Quality Supervision Test Institute Changchun 130000 P. R. China.
RSC Adv. 2020 Mar 6;10(17):9798-9807. doi: 10.1039/c9ra10405d.
Using agro-residues in bioreactors to produce mushrooms is an important component of eco-agriculture. Here, we tested the cultivation of with corn cob (CC) and corn straw (CS), and then evaluated the resulting enzyme activities, agronomic traits, textures and nutrient compositions of mushrooms. Laccase (T1 formula, 3.26 g U) and carboxymethyl cellulase (T3 formula, 1.01 g U) activities were the highest at the time for a complete substrate colonization stage (TCSC), while acidic xylanase activity was the highest (CK formula, 4.05 g U) in the mushroom block to color-turned (TMBCT) stage. The biological efficiency of growth on the T6 formula was 8.82% higher than growth on the CK formula, wherein the low C/N ratio of the substrate had an obvious negative effect on yield while the mass ratio of pileus (MRP) of fruiting bodies did not change with mixed substrates. No significant differences were observed in mineral composition for CK formulas, but corn crop waste (CCW) formulas exhibited more optimal nutritional contents. A formula containing more corn cob and sawdust (SD) (sum of at least 70%) as the substrate can produce fruiting bodies with good hardness. These results indicate that the use of corn cobs as the main ingredient, mixed with sawdust and corn straw to grow provides a more efficient use of agro-residues for growth. Thus, mixed agro-residue formulas have exceptional advantages in texture, nutrition of fruiting bodies, and yields.
利用生物反应器中的农业废弃物来生产蘑菇是生态农业的一个重要组成部分。在此,我们测试了用玉米芯(CC)和玉米秸秆(CS)栽培蘑菇,然后评估了所得蘑菇的酶活性、农艺性状、质地和营养成分。漆酶(T1配方,3.26 g U)和羧甲基纤维素酶(T3配方,1.01 g U)的活性在完全底物定殖阶段(TCSC)时最高,而酸性木聚糖酶活性在蘑菇菌块转色阶段(TMBCT)最高(CK配方,4.05 g U)。T6配方的生长生物学效率比CK配方高8.82%,其中底物的低碳氮比对产量有明显的负面影响,而子实体的菌盖质量比(MRP)不会随混合底物而变化。CK配方的矿物质组成没有显著差异,但玉米作物废弃物(CCW)配方表现出更优的营养成分。以含有更多玉米芯和锯末(SD)(总和至少70%)作为底物的配方可以生产出硬度良好的子实体。这些结果表明,以玉米芯作为主要成分,与锯末和玉米秸秆混合来栽培蘑菇能更有效地利用农业废弃物来促进生长。因此,并混合农业废弃物配方在质地、子实体营养和产量方面具有显著优势。