Laboratory for Forensic Chemistry, Department of Analytical Chemistry, Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, Poland.
Laboratory for Forensic Chemistry, Department of Analytical Chemistry, Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, Poland.
J Chromatogr B Analyt Technol Biomed Life Sci. 2019 Aug 15;1124:308-312. doi: 10.1016/j.jchromb.2019.06.025. Epub 2019 Jun 23.
The application of a new calculation strategy for the psychotropic drug concentration in blood and bone marrow samples in the form of dried blood spots (DBS) was the main aim of the study. The standard DBS method consists of the deposition of the capillary blood onto the dedicated paper cards. Nowadays, the DBS technique is seen as a fast and partly superior microsampling alternative methodology replacing the conventional venous blood and plasma collection. The calculation approach to drug concentration in the limited volume of the case sample, where the hematocrit level cannot be determined, constitutes an important step of this method. The method has been validated and the results of the determination of alprazolam and diazepam previously spiked in the post-mortem blood and bone marrow sample have been satisfactory.
本研究的主要目的是应用一种新的计算策略，用于检测血液和骨髓样本中以干血斑（DBS）形式存在的精神药物浓度。标准的 DBS 方法包括将毛细血管血沉积在专用的卡片纸上。如今，DBS 技术被视为一种快速且部分优于传统静脉采血和血浆采集的微采样替代方法。在无法确定红细胞压积水平的情况下，对有限体积的案例样本中的药物浓度进行计算，是该方法的重要步骤。该方法已经过验证，先前在死后血液和骨髓样本中添加的阿普唑仑和地西泮的测定结果令人满意。
