Alberto-Aguilar Dulce Rosario, Hernández-Ramírez Verónica Ivonne, Osorio-Trujillo Juan Carlos, Gallardo-Rincón Dolores, Toledo-Leyva Alfredo, Talamás-Rohana Patricia
Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Av. Instituto Politécnico Nacional 2508, Col. San Pedro Zacatenco, Delegación Gustavo A. Madero, 07360, Ciudad de México, Mexico.
Instituto Nacional de Cancerología, Av. San Fernando No. 22, Col. Sección XVI, Delegación Tlalpan, 07360, Ciudad de México, Mexico.
Cancer Microenviron. 2019 Dec;12(2-3):181-195. doi: 10.1007/s12307-019-00227-z. Epub 2019 Jul 2.
Ovarian cancer is considered to be the most lethal type of gynecological cancer. During the advanced stages of ovarian cancer, an accumulation of ascites is observed. Fucosylation has been classified as an abnormal post-translational modification that is present in many diseases, including ovarian cancer. Ovarian cancer cells that are cultured with ascites stimulation change their morphology; concomitantly, the fucosylation process is altered. However, it is not known which fucosylated proteins are modified. The goal of this work was to identify the differentially fucosylated proteins that are expressed by ovarian cancer cell lines that are cultured with ovarian cancer patients' ascites. Aleuria aurantia lectin was used to detect fucosylation, and some changes were observed, especially in the cell membrane. Affinity chromatography and mass spectrometry (MALDI-TOF) were used to identify 6 fucosylated proteins. Four proteins (Intermediate filament family orphan 1 [IFFO1], PHD finger protein 20-like protein 1 [PHF20L1], immunoglobulin gamma 1 heavy chain variable region partial [IGHV1-2], and Zinc finger protein 224 [ZNF224]) were obtained from cell cultures stimulated with ascites, and the other two proteins (Peregrin [BRPF1] and Dystrobrevin alpha [DTNA]) were obtained under normal culture conditions. The fucosylated state of some of these proteins was further analyzed. The experimental results show that the ascites of ovarian cancer patients modulated the fucosylation process. The PHD finger protein 20-like protein 1, Zinc finger protein 224 and Peregrin proteins colocalize with fucosylation at different levels.
卵巢癌被认为是最致命的妇科癌症类型。在卵巢癌晚期，会观察到腹水积聚。岩藻糖基化已被归类为一种异常的翻译后修饰，存在于包括卵巢癌在内的许多疾病中。用腹水刺激培养的卵巢癌细胞会改变其形态；同时，岩藻糖基化过程也会改变。然而，尚不清楚哪些岩藻糖基化蛋白被修饰。这项工作的目的是鉴定用卵巢癌患者腹水培养的卵巢癌细胞系所表达的差异岩藻糖基化蛋白。橙黄网柄牛肝菌凝集素用于检测岩藻糖基化，观察到了一些变化，尤其是在细胞膜中。采用亲和色谱和质谱（基质辅助激光解吸电离飞行时间质谱）来鉴定6种岩藻糖基化蛋白。从腹水刺激的细胞培养物中获得了4种蛋白（中间丝家族孤儿1 [IFFO1]、类PHD指蛋白20样蛋白1 [PHF20L1]、免疫球蛋白γ1重链可变区部分 [IGHV1-2] 和锌指蛋白224 [ZNF224]），另外两种蛋白（溴结构域包含蛋白1 [BRPF1] 和α-肌营养不良蛋白 [DTNA]）是在正常培养条件下获得的。对其中一些蛋白的岩藻糖基化状态进行了进一步分析。实验结果表明，卵巢癌患者的腹水调节了岩藻糖基化过程。类PHD指蛋白20样蛋白1、锌指蛋白224和溴结构域包含蛋白1在不同水平上与岩藻糖基化共定位。
