Department of General Surgery, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, PR China.
Clinical Surgery of Integrated Traditional Chinese and Western Medicine, Beijing University of Chinese Medicine, Beijing, PR China.
J Cosmet Dermatol. 2020 Feb;19(2):529-534. doi: 10.1111/jocd.13042. Epub 2019 Jul 2.
BACKGROUND & AIM: Panax notoginseng saponins are believed to promote wound healing due to its anti-proliferative effect on fibroblasts. The present work was therefore aimed to examine the beneficial effect of PNS on wound healing in vitro and in a murine model of cutaneous wound.
The in vitro effects of Panax notoginseng saponins on the proliferation of and nitric oxide (NO) synthesis in human fibroblast 3T3 cells were studied. The in vivo effects of Panax notoginseng saponins were examined in C57 mice with dorsal cutaneous wound. The healing rate and scar formation were followed after treatment with Panax notoginseng saponins. The histology and fibroblast accumulation in the wounds were studied using hematoxylin and eosin (H&E) staining. Expression of α-smooth muscle actin (α-SMA) was examined by immunohistochemistry.
Panax notoginseng saponins inhibited the proliferation of human fibroblast 3T3 with an EC of 1.825 mM Panax notoginseng saponins (0.1 mM) significantly promoted NO production (P < 0.01) and NO synthase activity (P < 0.01) of 3T3. In C57 mice with dorsal cutaneous wounds, 0.1 mM Panax notoginseng saponins significant expedited wound healing by reducing the size of lesions and suppressing the formation of scar. H&E staining revealed that treatment with Panax notoginseng saponins suppressed fibroblast accumulation in wound areas, while immunohistochemistry showed a significant reduction in α-SMA expression by 0.1 mM Panax notoginseng saponins.
Panax notoginseng saponins are a promising drug candidate that can accelerate wound healing and reduce scar formation.
三七总皂苷因其对成纤维细胞的抗增殖作用，被认为能促进伤口愈合。因此，本研究旨在体外和小鼠皮肤伤口模型中研究三七总皂苷对伤口愈合的有益作用。
研究了三七总皂苷对人成纤维细胞 3T3 增殖和一氧化氮（NO）合成的体外作用。在 C57 小鼠背部皮肤创伤中检测了三七总皂苷的体内作用。用三七总皂苷处理后，观察愈合率和瘢痕形成。用苏木精和伊红（H&E）染色研究伤口的组织学和成纤维细胞积累。用免疫组化法检测α-平滑肌肌动蛋白（α-SMA）的表达。
三七总皂苷以 1.825 mM 的 EC 抑制人成纤维细胞 3T3 的增殖（0.1 mM），显著促进 3T3 的 NO 产生（P < 0.01）和 NO 合酶活性（P < 0.01）。在 C57 小鼠背部皮肤创伤中，0.1 mM 的三七总皂苷通过减少病变大小和抑制瘢痕形成，显著加快伤口愈合。H&E 染色显示，用三七总皂苷处理可抑制伤口区域的成纤维细胞积累，而免疫组化显示 0.1 mM 的三七总皂苷可显著降低 α-SMA 的表达。
三七总皂苷是一种有前途的药物候选物，可加速伤口愈合，减少瘢痕形成。
