三七皂苷通过PI3K、AKT和ERK的磷酸化促进前交叉韧带损伤修复。
Panax notoginseng saponins promote wound repair of anterior cruciate ligament through phosphorylation of PI3K, AKT and ERK.
作者信息
Yu Lu, Xie Jingwei, Xin Na, Wang Zhanyou
机构信息
Department of Pathophysiology, College of Basic Medical Sciences, China Medical University Shenyang 110001, People's Republic of China ; Department of Rehabilitation Medicine, First Affiliated Hospital of China Medical University Shenyang 110001, People's Republic of China.
Department of Pathophysiology, College of Basic Medical Sciences, China Medical University Shenyang 110001, People's Republic of China.
出版信息
Int J Clin Exp Pathol. 2015 Jan 1;8(1):441-9. eCollection 2015.
Abstract
Panax notoginseng saponins (PNS) are components derived from Chinese herb panax notoginseng and play important roles in the cure of wounds. However, how PNS plays this function is still unclear. In this study, we used MTT assay, wound healing assay, western blot, quantitative real time PCR and enzyme-linked immunosorbent assay to detect the effects of PNS on the proliferation, migration and expression of collagen and fibronectin of anterior cruciate ligament (ACL) fibroblasts as well as the underlying mechanism. We found that PNS promoted the proliferation and migration of ACL fibroblasts and increased the expression levels of collagen and fibronectin. Further mechanism study indicates that PNS might play its function through the phosphorylation of PI3K, AKT and ERK. This study provides a possible mechanism for the function of PNS and lays foundation for further study on the function of panax notoginseng.
摘要
三七皂苷（PNS）是从中药材三七中提取的成分，在伤口愈合中发挥重要作用。然而，PNS如何发挥这一功能仍不清楚。在本研究中，我们采用MTT法、伤口愈合实验、蛋白质免疫印迹法、实时定量聚合酶链反应和酶联免疫吸附测定法，检测PNS对前交叉韧带（ACL）成纤维细胞增殖、迁移以及胶原蛋白和纤连蛋白表达的影响及其潜在机制。我们发现PNS促进了ACL成纤维细胞的增殖和迁移，并提高了胶原蛋白和纤连蛋白的表达水平。进一步的机制研究表明，PNS可能通过PI3K、AKT和ERK的磷酸化发挥其功能。本研究为PNS的功能提供了一种可能的机制，并为进一步研究三七的功能奠定了基础。
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