College of Life Sciences , Hebei Agricultural University , Baoding 071000 , China.
College of Plant Protection , Hebei Agricultural University , Baoding 071000 , China.
J Agric Food Chem. 2019 Jul 17;67(28):7908-7915. doi: 10.1021/acs.jafc.9b03038. Epub 2019 Jul 3.
In this study, to obtain higher agmatine yields using the previously developed strain AUX4 (JM109 Δ Δ Δ Δ), the genes encoding glutamate dehydrogenase (), glutamine synthetase (), phosphoenolpyruvate carboxylase (), aspartate aminotransferase (), transhydrogenase (), and biosynthetic arginine decarboxylase () were sequentially overexpressed by replacing their native promoters with the heterologous strong , core-, or 5Ps promoters to generate the plasmid-free strain AUX11. The fermentation results obtained using a 3-L bioreactor showed that AUX11 produced 2.93 g L agmatine with the yield of 0.29 g agmatine g glucose in the batch fermentation, and the fed-batch fermentation of AUX11 allowed the production of 40.43 g L agmatine with the productivity of 1.26 g L h agmatine. The results showed that the engineered strain AUX11 can be used for the industrial fermentative production of agmatine.
在这项研究中，为了使用先前开发的菌株 AUX4 (JM109 Δ Δ Δ Δ) 获得更高的胍丁胺产量，通过用异源强、核心或 5Ps 启动子替换其天然启动子，依次过表达编码谷氨酸脱氢酶 ()、谷氨酰胺合成酶 ()、磷酸烯醇丙酮酸羧激酶 ()、天冬氨酸转氨酶 ()、转氢酶 () 和生物合成精氨酸脱羧酶 () 的基因，生成无质粒的菌株 AUX11。使用 3-L 生物反应器进行发酵的结果表明，AUX11 在分批发酵中产生了 2.93 g L 的胍丁胺，其产量为 0.29 g 胍丁胺 g 葡萄糖，AUX11 的补料分批发酵允许生产 40.43 g L 的胍丁胺，其生产速率为 1.26 g L h agmatine。结果表明，工程菌株 AUX11 可用于胍丁胺的工业发酵生产。
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