纳诺吹：一种在纳升液相色谱-质谱联用中限制污染物的简单装置。

NanoBlow: A Simple Device To Limit Contaminants during NanoLC-MS.

机构信息

School of Life and Environmental Sciences, Charles Perkins Centre , University of Sydney , Sydney , NSW 2006 , Australia.

Sydney Mass Spectrometry , The University of Sydney , Sydney , NSW 2006 , Australia.

出版信息

J Proteome Res. 2019 Aug 2;18(8):3219-3222. doi: 10.1021/acs.jproteome.9b00175. Epub 2019 Jul 3.

DOI:10.1021/acs.jproteome.9b00175

PMID:31268342

Abstract

Limiting contamination of liquid chromatography/tandem mass spectrometry (LC-MS) systems and reducing the downtime associated with maintenance and cleaning is essential for productivity. We developed a simple device that creates a gas curtain barrier to prevent ions from entering the MS inlet. The gas can be quickly and easily applied when certain contaminant ions are known to elute. We show that the device can prevent the buildup of contaminants on the heated transfer capillary following >100 injections of a crude tissue lysate and improves peptide identifications. The device may provide a promising approach toward improving instrument robustness.

摘要

限制液相色谱/串联质谱（LC-MS）系统的污染并减少与维护和清洁相关的停机时间对于提高生产力至关重要。我们开发了一种简单的装置，该装置可以创建气体幕屏障，以防止离子进入 MS 入口。当已知某些污染物离子洗脱时，可以快速简便地应用气体。我们表明，该装置可以防止在对粗组织裂解物进行 >100 次注射后，在加热传输毛细管上积聚污染物，并提高肽的鉴定率。该装置可能为提高仪器稳定性提供了一种有前途的方法。