Martinez-Gutierrez Marlen, Arcila-Quiceno Victor, Trejos-Suarez Juanita, Ruiz-Saenz Julian
Universidad Cooperativa de Colombia, Grupo de Investigación en Ciencias Animales, Bucaramanga, Colombia.
Universidad de Santander, Facultad de Ciencias de la Salud, Programa de Bacteriología y Laboratorio Clínico, Grupo de Investigación en Manejo Clínico, Bucaramanga, Colombia.
Rev Inst Med Trop Sao Paulo. 2019 Jul 1;61:e34. doi: 10.1590/S1678-9946201961034.
After the introduction of the rotavirus vaccine, the number of rotavirus-associated deaths and the predicted annual rotavirus detection rate had slightly declined worldwide. Taking in account that in Colombia, Rotarix vaccine was introduced in 2009, the purpose of this study was to evaluate the presence of rotavirus A in children under five years who were treated for acute diarrhoea in Bucaramanga, Colombia and, moreover, to determine the genotypes of rotavirus present in those children. We performed an analytical cross-sectional study of rotavirus A in faecal samples from children up to five years of age. Stool samples were screened for rotavirus A using a lateral-flow immunochromatographic assay and confirmed using a VP6 sandwich ELISA. Genotyping of rotavirus A-positive samples was performed by PCR and sequencing of VP7 and VP4 genes. The overall prevalence of rotavirus was 30.53% (95% confidence interval [CI] 21.2 - 39.7). Most of the children with rotavirus (86.2%) had received two doses of the rotavirus vaccine. G3 strains accounted for the vast majority of cases (82.8%), followed by G12 strains (13.8%) and G3/G9 coinfections (3.4%). Among the P genotypes, P[8] was the most prevalent (69%), followed by P[9] (31%). The most common G[P] genotype combination was G3P[8], followed by G3P[9]. The main finding in this study was that rotavirus, in a Colombian region, is still an important pathogen in children under five years old, previously vaccinated. The results showed that different factors, such as kindergarten attendance, could explain the epidemiology and transmission of rotavirus in Bucaramanga.
在引入轮状病毒疫苗后，全球范围内与轮状病毒相关的死亡人数以及预计的年度轮状病毒检出率略有下降。考虑到在哥伦比亚，Rotarix疫苗于2009年引入，本研究的目的是评估在哥伦比亚布卡拉曼加因急性腹泻接受治疗的5岁以下儿童中A组轮状病毒的存在情况，此外，确定这些儿童中存在的轮状病毒基因型。我们对5岁以下儿童粪便样本中的A组轮状病毒进行了分析性横断面研究。使用侧向流动免疫层析法对粪便样本进行A组轮状病毒筛查，并使用VP6夹心ELISA进行确认。对A组轮状病毒阳性样本进行基因分型，通过PCR以及VP7和VP4基因测序来完成。轮状病毒的总体患病率为30.53%（95%置信区间[CI] 21.2 - 39.7）。大多数感染轮状病毒的儿童（86.2%）已接种两剂轮状病毒疫苗。G3毒株占绝大多数病例（82.8%），其次是G12毒株（13.8%）和G3/G9混合感染（3.4%）。在P基因型中，P[8]最为常见（69%），其次是P[9]（31%）。最常见的G[P]基因型组合是G3P[8]，其次是G3P[9]。本研究的主要发现是，在哥伦比亚的一个地区，轮状病毒在先前接种过疫苗的5岁以下儿童中仍然是一种重要的病原体。结果表明，诸如上幼儿园等不同因素可以解释布卡拉曼加轮状病毒的流行病学和传播情况。
