The Department of Pharmaceutical Engineering (State Key Laboratory of Functions and Applications of Medicinal Plants, the High Efficacy Application of Natural Medicinal Resources Engineering Center of Guizhou Province, the Key Laboratory of Optimal Utilization of Natural Medicine Resources), School of Pharmaceutical Sciences , Guizhou Medical University , University Town, Guian New District, Guizhou 550025 , China.
The Department of Pharmacology of Material Medical (High Educational Key Laboratory of Guizhou Province for Natural Medicinal Pharmacology and Drug Ability, the Union Key Laboratory of Guiyang City-Guizhou Medical University), School of Pharmaceutical Sciences , Guizhou Medical University , University Town, Guian New District, Guizhou 550025 , China.
Biomacromolecules. 2019 Aug 12;20(8):3031-3040. doi: 10.1021/acs.biomac.9b00598. Epub 2019 Jul 15.
Bacterial infections has become an intractable problem to human health. To overcome this challenge, we developed an antimicrobial agent (AgNPs@PDPE) via the conjugation of a pH-responsive copolymer of PDMAEMA--PPEGMA onto AgNPs surface. The AgNPs@PDPE underwent an acidity-induced surface charge conversion that favored bacteria-specific aggregation and antibacterial activity improvement. The specific interaction between AgNPs@PDPE and bacteria under acidic conditions was confirmed via an electrochemical method using AgNPs@PDPE modified glassy carbon electrode as the working electrode. AgNPs@PDPE could efficiently aggregate and inhibit the growth of both () and () under acidic condition. The AgNPs@PDPE could also selectively distinguish pathogenic bacteria from host cells, and this characteristic is benefical for reducing the damage to surrounding tissues in the host. Moreover, AgNPs@PDPE could promote the healing of - and -induced infection, as proven by the histological and TNF-α immunohistochemical analyses of rat dermal wounds. The proposed antimicrobial agent could to be an alternative treatment strategy for the safe treatment of treat bacteria-induced infections in clinics.
细菌感染已成为危害人类健康的一个棘手问题。为了克服这一挑战，我们通过将 PDMAEMA-PPEGMA 这一 pH 响应性共聚物接枝到 AgNPs 表面，开发了一种抗菌剂（AgNPs@PDPE）。AgNPs@PDPE 经历了酸度诱导的表面电荷转换，有利于细菌特异性聚集和抗菌活性的提高。通过使用 AgNPs@PDPE 修饰的玻碳电极作为工作电极的电化学方法，证实了 AgNPs@PDPE 与酸性条件下细菌之间的特异性相互作用。AgNPs@PDPE 可在酸性条件下有效聚集并抑制（）和（）的生长。AgNPs@PDPE 还可以选择性地区分病原菌和宿主细胞，这一特性有助于减少对宿主周围组织的损伤。此外，AgNPs@PDPE 可促进 - 和 - 诱导感染的愈合，这可通过大鼠皮肤创伤的组织学和 TNF-α 免疫组织化学分析得到证明。所提出的抗菌剂可以作为一种替代治疗策略，用于安全治疗临床上由细菌引起的感染。
