Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, People's Republic of China.
Mikrochim Acta. 2019 Jul 3;186(8):505. doi: 10.1007/s00604-019-3640-1.
A sensitive electrochemiluminescence (ECL) immunosensor was constructed for detection of insulin. It is based on the quenching effect of octahedral cuprous oxide decorated with gold nanoparticles (CuO@Au NPs). A composite constructed from multiwalled carbon nanotubes/reduced graphene oxide nanoribbons provides a large specific surface that promotes electron transfer on the surface of electrodes. In parallel, CdS nanoclusters were loaded with europium(III) to get CdS:Eu NCs. The Eu(III) ions exert a large enhancement in ECL intensity of the CdS NCs. CuO is biocompatible and has a large specific surface. It was used as a quencher for the ECL immunosensor. If Au NPs are loaded onto CuO, the green fluorescence of the CdS NCs (with emission peaks at 525 nm) is quenched. The CuO@Au NPs also can be employed as a label for the secondary antibodies (Ab) to improve the sensitivity of the ECL immunosensor. The assay works in the 0.5 pg·mL to 50 ng·mL insulin concentration range and has a detection limit of 40 fg·mL. Graphical abstract The multiwalled carbon nanotubes/reduced graphene oxide nanoribbons (MWCNT/rGONRs-CdS: Eu NCs) was used as substrate. Europium(III) (Eu) improves the ECL intensity of CdS NCs. The synergistic effect between CuO and Au NPs decreases the electrochemiluminescence of MWCNTs/rGONRs-CdS:Eu NCs.
一种灵敏的电化学发光（ECL）免疫传感器被构建用于检测胰岛素。它基于金纳米粒子修饰的八面体氧化亚铜（CuO@Au NPs）的猝灭效应。由多壁碳纳米管/还原氧化石墨烯纳米带组成的复合材料提供了一个大的比表面积，促进了电极表面的电子转移。同时，将硫化镉纳米簇负载铕（III）得到 CdS：Eu NCs。Eu（III）离子对 CdS NCs 的 ECL 强度有很大的增强作用。CuO 具有生物相容性和大的比表面积，被用作 ECL 免疫传感器的猝灭剂。如果将 Au NPs 负载到 CuO 上，则 CdS NCs 的绿色荧光（发射峰在 525nm 处）被猝灭。CuO@Au NPs 也可用作二次抗体（Ab）的标记物，以提高 ECL 免疫传感器的灵敏度。该测定法在 0.5pg·mL 至 50ng·mL 胰岛素浓度范围内有效，检测限为 40fg·mL。
