Gong Zhengxing, Shao Xinrong, Luo Jing, Sun Xu, Ma Hongmin, Wu Dan, Fan Dawei, Li Yuyang, Wei Qin, Ju Huangxian
Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, People's Republic of China.
Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, People's Republic of China.
Mikrochim Acta. 2023 Jan 19;190(2):59. doi: 10.1007/s00604-023-05635-z.
Based on the resonance energy transfer between CdS@CeO and CuO@PdAg, a quenching immunosensor for sensitive detection of prostate specific antigen (PSA) was constructed. The CdS@CeO heterostructure was obtained by in situ growth of CeO particles on the surface of CdS nanorods, and stable cathodic ECL emission was achieved using KSO as coreactant. CuO@PdAg was composed of CuO with tetradecahedral structure and bimetallic PdAg nanospheres and has a UV-V is absorption range between 600 and 800 nm. It overlaps with the ECL emission spectrum of CdS@CeO, realizing the effective quenching of the ECL signal, which provides feasibility for subsequent practical application. The immunosensor exhibited good linearity in the concentration range 10 fg·mL ~ 100 ng·mL, with a detection limit of 5.6 fg·mL. In sample analysis, the recoveries were 99.8-101%, and the relative standard deviation (RSD) was 0.85-1.6% showing great potential and development value for the sensitive detection of prostate cancer.
