用反向被动血凝试验检测曼氏血吸虫感染患者抗原刺激淋巴细胞培养上清液中的γ-干扰素。

Measurement of gamma-interferon in culture supernatants of antigen-stimulated lymphocytes from patients with Schistosoma mansoni infections by a reverse passive haemagglutination assay.

作者信息

Butterworth A E, Wilson A, Richardson B A, Harris J, Gachuhi R K, Senior D, Coombs R R

机构信息

Department of Pathology, University of Cambridge, UK.

出版信息

Clin Exp Immunol. 1988 Feb;71(2):241-6.

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1541446/

Abstract

An assay for gamma-interferon (IFN gamma) in human lymphocyte culture supernatants, based on reverse passive haemagglutination (RPH) of red cells bearing a monoclonal anti-IFN gamma antibody, was developed and compared with a conventional virus inhibition assay. Test samples comprised supernatants of lymphocytes from patients with Schistosoma mansoni infections, cultured with or without a soluble worm antigen preparation. The two assays gave comparable results, the correlations for individual samples being good. The RPH assay was both specific and sensitive, allowing the detection of IFN gamma at 13 u/ml (1 ng/ml) or less. The advantages of the RPH assay were that it was quick, relatively inexpensive and suitable for testing large numbers of samples. In particular, between-experiment variation was very low, allowing the assay of different samples on different occasions.

摘要

基于携带抗γ干扰素单克隆抗体的红细胞反向被动血凝反应（RPH），开发了一种检测人淋巴细胞培养上清液中γ干扰素（IFNγ）的方法，并与传统的病毒抑制试验进行了比较。测试样品包括来自曼氏血吸虫感染患者的淋巴细胞上清液，在有或没有可溶性虫抗原制剂的情况下进行培养。两种检测方法得出的结果相当，单个样品的相关性良好。RPH检测方法具有特异性和敏感性，能够检测出浓度为13 u/ml（1 ng/ml）或更低的IFNγ。RPH检测方法的优点是快速、相对便宜且适合检测大量样品。特别是，实验间的差异非常小，允许在不同时间对不同样品进行检测。

相似文献

1

Measurement of gamma-interferon in culture supernatants of antigen-stimulated lymphocytes from patients with Schistosoma mansoni infections by a reverse passive haemagglutination assay.用反向被动血凝试验检测曼氏血吸虫感染患者抗原刺激淋巴细胞培养上清液中的γ-干扰素。

Clin Exp Immunol. 1988 Feb;71(2):241-6.

2

The absence of gamma-interferon production of S. mansoni antigens in patients with schistosomiasis.

Braz J Med Biol Res. 1994 Jul;27(7):1619-25.

3

Are the cellular immune responses of children and adults with Schistosoma mansoni infection intrinsically different? Cytokines produced ex vivo in response to antigens and mitogens.曼氏血吸虫感染的儿童和成人的细胞免疫反应本质上是否不同？体外针对抗原和有丝分裂原产生的细胞因子。

Parasite Immunol. 2004 Jan;26(1):29-36. doi: 10.1111/j.0141-9838.2004.00680.x.

4

Recognition of different Schistosoma mansoni antigens by specific human T cell clones.特定人类T细胞克隆对不同曼氏血吸虫抗原的识别。

Cell Immunol. 1994 Mar;154(1):77-87. doi: 10.1006/cimm.1994.1058.

5

Serodiagnosis of Schistosoma mansoni infections in an endemic area of Burkina Faso: performance of several immunological tests with different parasite antigens.布基纳法索一个流行地区曼氏血吸虫感染的血清学诊断：几种使用不同寄生虫抗原的免疫学检测的性能

Acta Trop. 2005 Feb;93(2):169-80. doi: 10.1016/j.actatropica.2004.10.006. Epub 2004 Dec 23.

6

Human schistosomiasis mansoni: intensity of infection differentially affects the production of interleukin-10, interferon-gamma and interleukin-13 by soluble egg antigen or adult worm antigen stimulated cultures.人类曼氏血吸虫病：感染强度对可溶性虫卵抗原或成虫抗原刺激培养物中白细胞介素-10、干扰素-γ和白细胞介素-13的产生有不同影响。

Trans R Soc Trop Med Hyg. 2004 Sep;98(9):514-9. doi: 10.1016/j.trstmh.2003.11.009.

7

Identification of Schistosoma mansoni antigens recognized by T cells of C57BL/6 mice immunized with gamma-irradiated cercariae.鉴定用γ射线辐照尾蚴免疫的C57BL/6小鼠T细胞所识别的曼氏血吸虫抗原。

J Parasitol. 1994 Jun;80(3):421-31.

8

Cytokine production in acute versus chronic human Schistosomiasis mansoni: the cross-regulatory role of interferon-gamma and interleukin-10 in the responses of peripheral blood mononuclear cells and splenocytes to parasite antigens.急性与慢性曼氏血吸虫病患者体内细胞因子的产生：γ干扰素和白细胞介素-10在调节外周血单个核细胞和脾细胞对寄生虫抗原反应中的交叉调节作用

J Infect Dis. 1999 Jun;179(6):1502-14. doi: 10.1086/314748.

9

Substance P and somatostatin can modulate the amount of IgG2a secreted in response to schistosome egg antigens in murine schistosomiasis mansoni.P物质和生长抑素可调节曼氏血吸虫病小鼠体内针对血吸虫卵抗原分泌的IgG2a量。

J Immunol. 1993 Dec 15;151(12):6994-7004.

10

T-helper type-1-dominated lymph node responses induced in C57BL/6 mice by optimally irradiated cercariae of Schistosoma mansoni are down-regulated after challenge infection.在C57BL/6小鼠中，由曼氏血吸虫最佳照射尾蚴诱导的1型辅助性T细胞主导的淋巴结反应在攻击感染后下调。

Immunology. 1995 Feb;84(2):310-6.

本文引用的文献

1

Immunity after treatment of human schistosomiasis mansoni. I. Study design, pretreatment observations and the results of treatment.人类曼氏血吸虫病治疗后的免疫。I. 研究设计、治疗前观察及治疗结果

Trans R Soc Trop Med Hyg. 1984;78(1):108-23. doi: 10.1016/0035-9203(84)90190-1.

2

Use of monoclonal antibodies as sensitive and specific probes for biologically active human gamma-interferon.使用单克隆抗体作为生物活性人γ干扰素的灵敏且特异的探针。

Proc Natl Acad Sci U S A. 1984 Aug;81(16):5219-22. doi: 10.1073/pnas.81.16.5219.

3

Department of Biological Sciences, University of Warwick, Coventry, CV47AL, England.英国考文垂市华威大学生物科学系，邮编CV4 7AL

J Gen Virol. 1974 Dec;25(3):381-90. doi: 10.1099/0022-1317-25-3-381.

4

Immunity after treatment of human schistosomiasis mansoni. II. Identification of resistant individuals, and analysis of their immune responses.

Trans R Soc Trop Med Hyg. 1985;79(3):393-408. doi: 10.1016/0035-9203(85)90391-8.

5

Direct antibody rosette-forming reactions using monoclonal markers of lymphocyte subpopulations. Methodology and applications illustrated by investigations with rat pan-T antibodies of the CAMPATH series.

J Immunol Methods. 1986 Sep 27;92(2):241-9. doi: 10.1016/0022-1759(86)90172-9.

6

Harnessing the red cell for immunoassays.

Med Lab Sci. 1987 Jan;44(1):66-72.

7

Assays using red cell-labelled antibodies.使用红细胞标记抗体的检测方法。

J Immunol Methods. 1987 Jul 16;101(1):1-14. doi: 10.1016/0022-1759(87)90209-2.

8

Identity between human interferon-gamma and "macrophage-activating factor" produced by human T lymphocytes.人γ干扰素与人类T淋巴细胞产生的“巨噬细胞激活因子”的同一性。

Eur J Immunol. 1986 Dec;16(12):1471-7. doi: 10.1002/eji.1830161202.

9

Induction of protective immunity against Schistosoma mansoni by a nonliving vaccine. III. Correlation of resistance with induction of activated larvacidal macrophages.非活性疫苗诱导针对曼氏血吸虫的保护性免疫。III. 抗性与活化杀幼虫巨噬细胞诱导的相关性。

J Immunol. 1986 May 15;136(10):3872-7.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

文档翻译

学术文献翻译模型，支持多种主流文档格式。