Talmadge K W, Gallati H, Sinigaglia F, Walz A, Garotta G
Eur J Immunol. 1986 Dec;16(12):1471-7. doi: 10.1002/eji.1830161202.
Human peripheral blood monocytes purified by counterflow elutriation were activated in vitro by human natural or recombinant interferon-gamma (IFN-gamma) as shown by enhanced killing of Listeria monocytogenes and increased production of H2O2 in response to phorbol myristate acetate. Half-maximal stimulation for macrophage activation (MAF) was observed with 10-20 antiviral U/ml of purified recombinant IFN-gamma. These MAF activities were found to correlate with the antiviral activity dependent on IFN-gamma under several experimental conditions. Both activities were recovered together from supernatants of concanavalin A-stimulated peripheral blood mononuclear cells and in the media of a large number of T cell clones of different specificities. The parallelism between the two activities was also observed upon fractionation of culture media from producing cells and upon treatment of such preparations with low pH and high temperature. Finally, three antibodies with different specificities were found to abrogate the MAF and antiviral activities from lymphocyte culture supernatant. These results indicate that MAF released by stimulated lymphocytes is identical to IFN-gamma.
通过逆流淘析纯化的人外周血单核细胞，经人天然或重组干扰素-γ（IFN-γ）体外激活，表现为对单核细胞增生李斯特菌的杀伤增强以及对佛波酯肉豆蔻酸酯乙酸酯反应时过氧化氢生成增加。用10 - 20抗病毒单位/毫升的纯化重组IFN-γ可观察到巨噬细胞激活（MAF）的半数最大刺激。在几种实验条件下，发现这些MAF活性与依赖IFN-γ的抗病毒活性相关。两种活性都能从伴刀豆球蛋白A刺激的外周血单核细胞上清液以及大量不同特异性的T细胞克隆培养基中共同回收。在对产生细胞的培养基进行分级分离以及用低pH和高温处理此类制剂时，也观察到了两种活性之间的平行关系。最后，发现三种具有不同特异性的抗体可消除淋巴细胞培养上清液中的MAF和抗病毒活性。这些结果表明，受刺激淋巴细胞释放的MAF与IFN-γ相同。
