Mutchnick M G, Lee H H, Hollander D I, Haynes G D, Chua D C
Department of Internal Medicine, Wayne State University School of Medicine, Detroit, Michigan 48201.
Clin Immunol Immunopathol. 1988 Apr;47(1):84-92. doi: 10.1016/0090-1229(88)90147-x.
Gamma interferon (IFN-gamma) production by peripheral blood mononuclear cells (PBM) was measured in 21 patients with Crohn's disease, in 15 patients with ulcerative colitis, in 12 patients with non-IBD gastrointestinal disease (disease control), and in 28 healthy controls. T-cell subset proportions and serum levels of thymosin alpha 1 and thymosin beta 4, two hormonelike thymic peptides, were also determined. No differences were seen in T-cell subset proportions in patients with Crohn's disease or ulcerative colitis when compared to healthy controls or to the disease-control group. In vitro IFN-gamma production was markedly decreased in Crohn's disease and in untreated, but not treated, patients with ulcerative colitis. Preincubation of PBM prior to the addition of inducer mitogen resulted in enhanced IFN-gamma production in patients with Crohn's disease or ulcerative colitis which significantly exceeded that seen either in healthy controls or in the disease-control group. Serum thymosin alpha 1 levels were comparable in all study groups; however, serum thymosin beta 4 concentrations were significantly higher in all patient groups than in the healthy controls. These results confirm a defective in vitro IFN-gamma production in patients with IBD which is apparently independent of endocrine thymus regulation.
对21例克罗恩病患者、15例溃疡性结肠炎患者、12例非炎症性肠病（IBD）胃肠道疾病患者（疾病对照组）及28例健康对照者，检测其外周血单个核细胞（PBM）产生γ干扰素（IFN-γ）的情况。还测定了两种类激素胸腺肽——胸腺素α1和胸腺素β4的T细胞亚群比例及血清水平。与健康对照者或疾病对照组相比，克罗恩病或溃疡性结肠炎患者的T细胞亚群比例未见差异。克罗恩病患者及未治疗（而非已治疗）的溃疡性结肠炎患者的体外IFN-γ产生明显减少。在添加诱导有丝分裂原之前对PBM进行预孵育，可使克罗恩病或溃疡性结肠炎患者的IFN-γ产生增强，且显著超过健康对照者或疾病对照组。所有研究组的血清胸腺素α1水平相当；然而，所有患者组的血清胸腺素β4浓度均显著高于健康对照者。这些结果证实IBD患者存在体外IFN-γ产生缺陷，这显然与胸腺内分泌调节无关。
