Mes-Masson A M, McLaughlin J, Witte O
Molecular Biology Institute, University of California, Los Angeles 90024.
Haematol Blood Transfus. 1987;31:163-6. doi: 10.1007/978-3-642-72624-8_35.
The reciprocal translocation between human chromosomes 9 and 22, termed the Philadelphia chromosome (Ph1), is observed in more than 90% of patients with chronic myelogenous leukemia. This translocation fuses sequences from a variable distance 5' to the c-abl locus on chromosome 9 to sequences in a breakpoint cluster region (bcr) on chromosome 22. The appearance of the Ph1 chromosome is correlated with the production of a novel 8.7-kb RNA transcript containing both bcr and c-abl sequences as well as with a 210-kd phosphoprotein (p210c-abl) representing non-abl polypeptide sequences fused to c-abl-derived sequences. Antibodies prepared to a number of different c-abl domains and to bcr determinants were employed to characterize the normal and altered c-abl gene products. By combining a variety of cDNA cloning techniques, we have isolated bcr/abl clones representing 8.7 kb of contiguous mRNA sequence.
在超过90%的慢性粒细胞白血病患者中可观察到人类9号和22号染色体之间的相互易位，即所谓的费城染色体（Ph1）。这种易位将9号染色体上c-abl基因座5'端可变距离处的序列与22号染色体上一个断裂点簇区域（bcr）中的序列融合。Ph1染色体的出现与一种包含bcr和c-abl序列的新型8.7 kb RNA转录本的产生以及一种210 kd磷蛋白（p210c-abl）相关，该磷蛋白代表与c-abl衍生序列融合的非abl多肽序列。针对多个不同c-abl结构域和bcr决定簇制备的抗体被用于鉴定正常和改变的c-abl基因产物。通过结合多种cDNA克隆技术，我们分离出了代表8.7 kb连续mRNA序列的bcr/abl克隆。
