Lee M S, Chang K S, Freireich E J, Kantarjian H M, Talpaz M, Trujillo J M, Stass S A
Division of Laboratory Medicine, University of Texas System Cancer Center, Houston 77030.
Blood. 1988 Sep;72(3):893-7.
The Philadelphia (Ph1) chromosome in chronic myelogenous leukemia (CML) involves reciprocal translocation of the bcr gene and the c-abl oncogene. The fused bcr/abl gene is transcribed into two types of chimeric mRNA. By means of a combined method of S1 nuclease protection and polymerase chain reaction, we amplified sequences representative of the chimeric bcr/abl transcripts. Only 5 micrograms of total cellular RNA is needed and the chimeric bcr/abl message can be detected at a dilution of 1:100,000. We also detected residual chimeric bcr/abl transcripts in the remission samples from two Ph1-positive CML patients. This technique has the potential to identify a subpopulation of Ph1-positive CML patients in remission who are at high risk of relapse.
慢性粒细胞白血病（CML）中的费城（Ph1）染色体涉及bcr基因和c-abl癌基因的相互易位。融合的bcr/abl基因转录成两种类型的嵌合mRNA。通过S1核酸酶保护和聚合酶链反应的联合方法，我们扩增了代表嵌合bcr/abl转录本的序列。仅需要5微克总细胞RNA，并且可以在1:100,000的稀释度下检测到嵌合bcr/abl信息。我们还在两名Ph1阳性CML患者的缓解样本中检测到残留的嵌合bcr/abl转录本。该技术有可能识别出处于缓解期但复发风险高的Ph1阳性CML患者亚群。
