Kunihiro Satoru, Tanabe Daigo, Niwa Yuiko, Kitamura Keisuke, Abe Jun, Yamada Tetsuya
Graduate School of Agriculture, Hokkaido University, Sapporo, Hokkaido 060-8589, Japan.
School of Agriculture, Hokkaido University, Sapporo, Hokkaido 060-8589, Japan.
Plant Biotechnol (Tokyo). 2017;34(1):45-49. doi: 10.5511/plantbiotechnology.16.1215a. Epub 2017 Feb 11.
We isolated an ortholog () of the Arabidopsis R2R3-MYB transcription factor (TF) gene from to investigate the regulation of flavonoid biosynthesis, which is driven by many paralogous genes in . We characterized the spatial and temporal expression of in leaves, stems, roots, flowers, immature seeds, seedling leaves, and seedling roots. Expression was much higher in flowers than in other tissues. To verify the relationship between the expression of and that of flavonoid biosynthesis genes, we generated transgenic plants overexpressing . Overexpression of resulted in the upregulation of genes for a chalcone synthase paralog (), flavanone 3-hydroxylase, and flavonol synthase. Interestingly, LjMYB12 strongly activated but did not activate other paralogs. This result suggests differences in the spatial or temporal activation of paralogs by R2R3-MYB TFs. Molecular characterization of R2R3-MYB TFs in will reveal the effects of gene duplication on the regulation of diverse flavonoid biosynthesis.
我们从[植物名称]中分离出拟南芥R2R3-MYB转录因子（TF）基因的一个直系同源基因（[基因名称]），以研究黄酮类生物合成的调控，该生物合成由[植物名称]中的许多旁系同源基因驱动。我们对[基因名称]在叶片、茎、根、花、未成熟种子、幼苗叶片和幼苗根中的时空表达进行了表征。其在花中的表达远高于其他组织。为了验证[基因名称]的表达与黄酮类生物合成基因表达之间的关系，我们构建了过表达[基因名称]的转基因[植物名称]植株。[基因名称]的过表达导致查尔酮合酶旁系同源基因（[基因名称]）、黄烷酮3-羟化酶和黄酮醇合酶基因的上调。有趣的是，LjMYB12强烈激活[基因名称]，但不激活其他[基因名称]旁系同源基因。这一结果表明R2R3-MYB转录因子对[基因名称]旁系同源基因的时空激活存在差异。对[植物名称]中R2R3-MYB转录因子的分子表征将揭示基因复制对多种黄酮类生物合成调控的影响。
