Li Gang, Zhang Daohai, Qin Shuhao
College of Materials Science and Metallurgy Engineering, Guizhou University, Guiyang 550025, China.
National Engineering Research Center for Compounding and Modification of Polymeric Materials, National and Local Joint Engineering Research Center for Functional Polymer Membrane Materials and Membrane Processes, Guiyang 550014, China.
Nanomaterials (Basel). 2019 Jul 3;9(7):972. doi: 10.3390/nano9070972.
Silver nano-particles (AgNPs)-filled antibacterial materials have been widely employed in the fields of biology and biomedicine. However, AgNPs have shown obvious cytotoxicity. Hence it is more reasonable to use silver chloride nanoparticles (AgCl NPs) to prepare antibacterial materials due to the slow release of silver ions created by AgCl NPs formed in the chitosan. In this experiment, a useful antibacterial hydrogel for skin repairation was prepared by exploring the relationship between AgCl NPs and cytotoxicity. It is worth noting that the crosslinked network structure was successfully obtained in an antibacterial AgCl/PVA (Polyvinyl alcohol)/PEG (Polyethylene glycol)/CS (Chitosan) hydrogel materials by the hydrothermal method. In detail, the dynamic particle size distribution of AgCl NPs was relatively uniform, which is analyzed by a dynamic light scattering (DLS). The internal structure of the lyophilized hydrogel showed obvious porous structure, indicating that the hydrogel had high water content. The result of X-ray photoelectron spectroscopy (XPS) confirmed the existence of a silver element. The release concentration of silver ions was analyzed by inductively coupled plasma (ICP) to study the effect of silver ions release concentration on the antibacterial activity and cytotoxicity of hydrogel. The results show that the lower concentration of silver ions can make the hydrogel have good antibacterial activity and low cytotoxicity. The bacteriostatic rate of the antibacterial hydrogel was over 90%. Simultaneously, the mechanical properties test shows that the hydrogel has good mechanical properties, which can be widely used as an antibacterial material.
填充银纳米颗粒（AgNPs）的抗菌材料已在生物学和生物医学领域广泛应用。然而，AgNPs已显示出明显的细胞毒性。因此，由于壳聚糖中形成的AgCl NPs产生的银离子缓慢释放，使用氯化银纳米颗粒（AgCl NPs）制备抗菌材料更为合理。在本实验中，通过探索AgCl NPs与细胞毒性之间的关系，制备了一种用于皮肤修复的有用抗菌水凝胶。值得注意的是，通过水热法在抗菌AgCl/聚乙烯醇（PVA）/聚乙二醇（PEG）/壳聚糖（CS）水凝胶材料中成功获得了交联网络结构。详细地说，通过动态光散射（DLS）分析，AgCl NPs的动态粒径分布相对均匀。冻干水凝胶的内部结构显示出明显的多孔结构，表明该水凝胶具有高含水量。X射线光电子能谱（XPS）结果证实了银元素的存在。通过电感耦合等离子体（ICP）分析银离子的释放浓度，以研究银离子释放浓度对水凝胶抗菌活性和细胞毒性的影响。结果表明，较低浓度的银离子可使水凝胶具有良好的抗菌活性和低细胞毒性。抗菌水凝胶的抑菌率超过90%。同时，力学性能测试表明该水凝胶具有良好的力学性能，可广泛用作抗菌材料。
