College of Life Science, Shandong Normal University, Jinan, 250014, Shandong, China.
College of Life Science, Shandong Normal University, Jinan, 250014, Shandong, China.
Biochem Biophys Res Commun. 2019 Aug 27;516(3):969-975. doi: 10.1016/j.bbrc.2019.06.138. Epub 2019 Jul 2.
The TFIIB-related factor (BRF) family plays vital roles in RNA polymerase (Pol) III transcription initiation. However, little is known about the role of BRF in plants. Here, we report BRF1 and BRF2 are involved in Arabidopsis reproduction. In this study, we generated BRF1 and BRF2 double mutant plants. We found that no homozygous double mutants of brf1brf2 were obtained when brf1 and brf2 were crossed, although brf1 and brf2 mutants individually developed and reproduced normally. Further experiments revealed that heterozygous brf1/ + brf2/ + produced abnormal pollen and had no seeds in some placentas of siliques. Genetic data derived from reciprocal crosses showed that BRF2 plays a dominant role in Arabidopsis reproduction. Taken together, a double mutation of BRF1 and BRF2 results in a high degree of aborted macrogametes and microgametes and complete failure in zygote generation, ultimately leading to sterility.
TFIIB 相关因子（BRF）家族在 RNA 聚合酶（Pol）III 转录起始中发挥重要作用。然而，人们对植物中 BRF 的作用知之甚少。在这里，我们报告 BRF1 和 BRF2 参与拟南芥的繁殖。在这项研究中，我们生成了 BRF1 和 BRF2 双突变体植物。我们发现，当 brf1 和 brf2 杂交时，没有获得纯合双突变体 brf1brf2，尽管 brf1 和 brf2 突变体个体发育和繁殖正常。进一步的实验表明，杂合 brf1/+ brf2/+ 产生异常花粉，并且在一些子房的胎座中没有种子。来自正反交的遗传数据表明，BRF2 在拟南芥的繁殖中起主导作用。总之，BRF1 和 BRF2 的双突变导致大量大配子和小配子的高度退化，以及合子生成的完全失败，最终导致不育。
