使用快速冷冻法I对薮猫（Leptailurus serval）和兔狲（Felis manul）卵母细胞进行冷冻保存后的活力
The Viability of Serval (Leptailurus serval) and Pallas Cat (Felis manul) Oocytes after Cryopreservation Using the Rapid-I Method.
作者信息
Nowak A, Kochan J, Prochowska S, Partyka A, Młodawska W, Witarski W, Skotnicki J, Grega T, Pałys M, Niżański W
机构信息
University of Agriculture in Krakow, Faculty of Animal Sciences, Institute of Veterinary Sciences, al. Mickiewicza 21, 30-120 Krakow, Poland.
Wrocław University of Environmental and Life Sciences, Faculty of Veterinary Medicine, Department of Reproduction and Clinic of Farm Animals, Poland.
出版信息
Cryo Letters. 2019 Jul/Aug;40(4):226-230.
BACKGROUND
Vitrification by Rapid-I method could be essential for felid rescue programs to protect wild felid in the future.
OBJECTIVE
This study was aimed at adapting the Rapid I method and evaluating the viability of serval and Pallas cat oocytes compared to oocytes of the domestic cat.
MATERIALS AND METHODS
Oocytes after collection and in vitro maturation were vitrified using Cryotech medium (Cryotech, Japan) and a Rapid-I device (Vitrolife, Sweden). To evaluate viability, oocytes after warming were stained with fluorescein diacetate and ethidium bromide.
RESULTS
Survival rate in the control group (domestic cat) was 75 %. In the experimental group, 70% (serval) and 60% (pallas cat) viable oocytes were found.
CONCLUSION
The Rapid-I method can be applied successfully for the vitrification of wild felid oocytes.
背景
快速I法玻璃化冷冻对于未来野生猫科动物救援计划中保护野生猫科动物可能至关重要。
目的
本研究旨在采用快速I法，并比较薮猫和兔狲卵母细胞与家猫卵母细胞的活力。
材料与方法
采集并体外成熟后的卵母细胞使用Cryotech培养基（日本Cryotech公司）和快速I设备（瑞典Vitrolife公司）进行玻璃化冷冻。为评估活力，解冻后的卵母细胞用二乙酸荧光素和溴化乙锭染色。
结果
对照组（家猫）的存活率为75%。在实验组中，发现70%（薮猫）和60%（兔狲）的卵母细胞具有活力。
结论
快速I法可成功应用于野生猫科动物卵母细胞的玻璃化冷冻。
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