Formula-Pattern Research Center, School of Traditional Chinese Medicine, Jinan University, Guangzhou, China.
Department of Musculoskeletal Oncology, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China.
Int J Clin Oncol. 2019 Nov;24(11):1479-1489. doi: 10.1007/s10147-019-01500-3. Epub 2019 Jul 5.
We investigated the microarray data GSE42352 to identify genes that can be used as prognosis factors in osteosarcoma.
Gene Ontology (GO) biological process analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of Cytoscape ClueGo were used in verifying the function of different genes. Realtime-PCR were used to confirm the microarray results. 83 patient samples were collected and underwent Kaplan-Meier survival analysis and multivariate analysis to predict the prospect of genes using as prognosis factors.
After analyzing the microarray data GSE42352, mitosis metaphase to anaphase-related genes CDC20, securin, cyclin A2 and cyclin B2 were found to be overexpressed in osteosarcoma cell lines. Kaplan-Meier survival analysis showed that overexpression of these genes can predict poor prognosis outcomes in osteosarcoma patients. Furthermore, any combination of the four genes seems to be more effective in predicting osteosarcoma outcomes than any of these genes alone.
CDC20 and its downstream substracts securin, cyclin A2 and cyclin B2 are good factors that can predict prognosis outcomes in osteosarcoma. Any two combination of these four genes are more effective to be used as osteosarcoma prognosis factors.
我们研究了微阵列数据 GSE42352，以鉴定可作为骨肉瘤预后因素的基因。
使用 Cytoscape ClueGo 的基因本体论（GO）生物过程分析和京都基因与基因组百科全书（KEGG）分析来验证不同基因的功能。实时-PCR 用于验证微阵列结果。收集了 83 例患者样本，进行 Kaplan-Meier 生存分析和多变量分析，以使用预后因素预测基因的前景。
分析微阵列数据 GSE42352 后，发现有丝分裂中期到后期相关基因 CDC20、securin、cyclin A2 和 cyclin B2 在骨肉瘤细胞系中过表达。Kaplan-Meier 生存分析表明，这些基因的过表达可以预测骨肉瘤患者的预后不良。此外，这四个基因中的任何组合似乎比单独使用这些基因中的任何一个都更能有效地预测骨肉瘤的结果。
CDC20 及其下游亚基 securin、cyclin A2 和 cyclin B2 是预测骨肉瘤预后结果的良好因素。这四个基因中的任何两个组合都更有效地用作骨肉瘤预后因素。
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