Kober S Liane, Hollert Henner, Frohme Marcus
Molecular Biotechnology and Functional Genomics, Technical University of Applied Sciences Wildau, Hochschulring 1, 15745, Wildau, Germany.
Institute for Environmental Research (Biology V), Department of Ecosystem Analysis, RWTH Aachen University, Worringerweg 1, 52074, Aachen, Germany.
Anal Bioanal Chem. 2019 Sep;411(23):5993-6003. doi: 10.1007/s00216-019-01976-y. Epub 2019 Jul 5.
Contamination from various sources is a global environmental and health threat, with mining and military activities in particular having spread nitroaromatic compounds, such as 2,4,6-trinitrotoluene and its degradation products and by-products, to the soil. The investigation and monitoring of large contaminated areas requires new detection methods since the established ones are expensive and time-consuming. Hence, we established a matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) method using 1,5-diaminonaphthalene as the matrix substance and an internal standard for quantification. Analyzing standard substances, we found specific signals for radical and fragment ions of different nitrotoluenes and nitrobenzenes with good reproducibility and detection limits down to 0.25 ng/μL. The analysis of soil sample extracts from a former production site showed clear signals for 2,4,6-trinitrotoluene and the primary degradation products aminodinitrotoluenes. Furthermore, quantification gave results comparable to those obtained by conventional liquid chromatography-tandem mass spectrometry analysis. The MALDI-TOF MS method has a comparatively lower reproducibility, with relative standard deviations of 6% to 20% for multiple measurements of standard solutions and soil sample extracts. Nevertheless, a comparison of both methods revealed the advantages of MALDI-TOF MS analysis of explosive-contaminated areas with regard to costs, time, and handling. Finally, our MALDI-TOF MS method fulfills all the needs for high sample throughput and can therefore be a valuable screening tool for explosive-contaminated areas. Graphical abstract.
来自各种来源的污染是全球环境和健康威胁，尤其是采矿和军事活动已将硝基芳香化合物，如2,4,6-三硝基甲苯及其降解产物和副产物扩散到土壤中。由于既定的检测方法昂贵且耗时，对大面积污染区域的调查和监测需要新的检测方法。因此，我们建立了一种基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）方法，使用1,5-二氨基萘作为基质物质和内标进行定量。分析标准物质时，我们发现了不同硝基甲苯和硝基苯的自由基和碎片离子的特定信号，具有良好的重现性，检测限低至0.25 ng/μL。对一个 former生产 site的土壤样品提取物的分析显示了2,4,6-三硝基甲苯和主要降解产物氨基二硝基甲苯的清晰信号。此外，定量结果与通过传统液相色谱-串联质谱分析获得的结果相当。MALDI-TOF MS方法的重现性相对较低，标准溶液和土壤样品提取物多次测量的相对标准偏差为6%至20%。然而，两种方法的比较揭示了MALDI-TOF MS分析爆炸物污染区域在成本、时间和操作方面的优势。最后，我们的MALDI-TOF MS方法满足了高样品通量的所有需求，因此可以成为爆炸物污染区域的有价值的筛选工具。图形摘要。
原文中“former production site”这里“former”翻译为“以前的”，“site”翻译为“场地”，整体可理解为“以前的生产场地”，但不太明确“former”具体所指，所以翻译可能稍显生硬，你可根据实际情况调整。
