Department of Environmental & Biological Chemistry, Chungbuk National University, Cheongju, Chungbuk 28644, Republic of Korea.
Department of Energy and Resources Engineering, Chonnam National University, Gwangju 61186, Republic of Korea.
J Hazard Mater. 2019 Nov 5;379:120825. doi: 10.1016/j.jhazmat.2019.120825. Epub 2019 Jun 26.
Tungsten is an emerging contaminant because of its potential toxicity to humans. However, tungsten-plant-microbe interactions remains unknown. The objective of the study was to evaluate the effect of tungsten-resistant bacteria on tungsten species in plants and microbial community structure in soil. Although bacterial inoculation did not affect lettuce (Lactuca sativa L.) growth or tungsten uptake via root, tungsten-resistant bacteria increased translocation of tungsten from root to shoot. Bacterial inoculation slightly oxidized tungsten in lettuce based on tungsten L x-ray absorption near-edge structure (XANES). Tungsten in lettuce roots and shoots grown in tungsten(VI)-spiked soil existed as a mixture of tungsten(IV) and tungsten(VI). Tungsten accumulated as polytungstate in the root and monotungstate in the shoot. Inoculation with tungsten-resistant bacteria and plant growth increased microbial diversity in tungsten-contaminated soil. In tungsten-spiked soils without plants, metal-resistant or reducing bacteria were found while bacteria growing in rhizosphere were detected in soils supporting plant growth. These results indicate a role of the bacteria and plants in phytoremediation of tungsten-contaminated soil.
由于其对人类潜在的毒性，钨是一种新兴的污染物。然而，关于钨-植物-微生物的相互作用仍然未知。本研究的目的是评估耐钨细菌对植物中钨形态和土壤微生物群落结构的影响。虽然细菌接种并未通过根影响生菜（Lactuca sativa L.）的生长或钨的吸收，但耐钨细菌增加了钨从根到茎的转运。基于钨 L 射线吸收近边结构（XANES），细菌接种使生菜中的钨略微氧化。在添加六价钨的土壤中生长的生菜根和茎中的钨以钨（IV）和钨（VI）混合物的形式存在。钨在根中积累为多钨酸盐，在茎中积累为一钨酸盐。耐钨细菌和植物生长的接种增加了受钨污染土壤中的微生物多样性。在没有植物的添加六价钨土壤中，发现了耐金属或还原细菌，而在支持植物生长的土壤中则检测到根际细菌。这些结果表明细菌和植物在受钨污染土壤的植物修复中发挥作用。
