Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143, USA; Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus, Denmark.
Department of Physiology, University of California, San Francisco, CA 94143, USA.
Curr Opin Struct Biol. 2019 Oct;58:259-268. doi: 10.1016/j.sbi.2019.05.022. Epub 2019 Jul 3.
Advances in electron microscopes, detectors and data processing algorithms have greatly facilitated the structural determination of many challenging integral membrane proteins that have been evasive to crystallization. These breakthroughs facilitate the application and development of various membrane protein solubilization approaches for structural studies, including reconstitution into lipid nanoparticles. In this review, we discuss various approaches for preparing transmembrane proteins for structural determination with single-particle electron cryo microscopy (cryoEM).
电子显微镜、探测器和数据处理算法的进步极大地促进了许多具有挑战性的整合膜蛋白的结构测定,这些蛋白一直难以结晶。这些突破促进了各种膜蛋白溶解方法在结构研究中的应用和发展,包括重构成脂质纳米颗粒。在这篇综述中,我们讨论了用于通过单颗粒电子冷冻电镜(cryoEM)进行结构测定的各种跨膜蛋白制备方法。