Zhejiang Provincial Center for Disease Control and Prevention, 3399 Bin Sheng Road, Binjiang District, Hangzhou 310051, China.
Zhejiang Provincial Center for Disease Control and Prevention, 3399 Bin Sheng Road, Binjiang District, Hangzhou 310051, China.
Sci Total Environ. 2019 Nov 1;689:937-944. doi: 10.1016/j.scitotenv.2019.06.425. Epub 2019 Jun 26.
This study aimed to evaluate the body burdens of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) and their associated health impacts toward school-age children living near a municipal waste incinerator (MWI). A total of 82 children from the exposure area and 49 from the control area were recruited. We measured blood PCDD/F levels, conducted comet assays, calculated the percentage of 5-methylcytosine (%5-mC) and 5-hydroxymethylcytosine (%5-hmC), performed flow cytometry, measured hormonal levels, and analyzed hematological parameters. We also examined 17 congeners of PCDD/Fs in environmental samples, namely, eggs, rice, water, soil, and PM The mean blood levels of ΣPCDD/Fs and TEQ-ΣPCDD/Fs were statistically higher in the exposure group than in the control group (3.40 vs. 2.77 pg/g wet weight and 0.40 vs. 0.28 pg WHO-TEQ/g wet weight, respectively; p < 0.05). By contrast, the %5-mC and %5-hmC levels were statistically lower in the exposure group than in the control group (1.15% vs. 4.66% and 0.22% vs. 0.30%, respectively; p < 0.01), whereas the mean % tail DNA was statistically higher in the exposure group than in the control group (10.10% vs. 8.28%, p < 0.01). The mean blood levels of ΣPCDD/Fs and TEQ-ΣPCDD/Fs were both negatively correlated with %5-mC (r = -0.245 and r = -0.217, respectively; p < 0.01) but not with %5-hmC and % tail DNA (p > 0.05). Furthermore, the mean ΣPCDD/F levels in eggs and soil obtained from the exposure area were statistically higher than those of the samples obtained from the control area (31.08 vs. 4.32 pg/g dry weight and 1026.04 vs. 674.97 pg/g dry weight, respectively). In conclusion, children living near the MWI may suffer genetic and epigenetic modifications, such as DNA damage or global DNA hypomethylation due to the MWI-emitted PCDD/Fs and other contaminants.
本研究旨在评估居住在城市垃圾焚烧厂（MWI）附近的学龄儿童体内多氯二苯并对二恶英和呋喃（PCDD/Fs）的负担及其相关健康影响。共招募了来自暴露区的 82 名儿童和来自对照区的 49 名儿童。我们测量了血液 PCDD/F 水平，进行了彗星试验，计算了 5-甲基胞嘧啶（%5-mC）和 5-羟甲基胞嘧啶（%5-hmC）的百分比，进行了流式细胞术，测量了激素水平，并分析了血液学参数。我们还检测了环境样本中的 17 种 PCDD/F 同系物，即鸡蛋、大米、水、土壤和 PM。暴露组血液中ΣPCDD/Fs 和 TEQ-ΣPCDD/Fs 的平均水平明显高于对照组（分别为 3.40 与 2.77 pg/g 湿重和 0.40 与 0.28 pg WHO-TEQ/g 湿重；p<0.05）。相比之下，暴露组 %5-mC 和 %5-hmC 的水平明显低于对照组（分别为 1.15%与 4.66%和 0.22%与 0.30%；p<0.01），而暴露组平均%尾 DNA 明显高于对照组（10.10%与 8.28%；p<0.01）。ΣPCDD/Fs 和 TEQ-ΣPCDD/Fs 的平均血液水平均与%5-mC 呈负相关（r=-0.245 和 r=-0.217；p<0.01），但与%5-hmC 和%尾 DNA 无相关性（p>0.05）。此外，暴露区鸡蛋和土壤中ΣPCDD/F 的平均水平明显高于对照区（分别为 31.08 与 4.32 pg/g 干重和 1026.04 与 674.97 pg/g 干重）。总之，由于 MWI 排放的 PCDD/Fs 和其他污染物，居住在 MWI 附近的儿童可能会遭受遗传和表观遗传改变，例如 DNA 损伤或全基因组 DNA 低甲基化。
