Graduate School of Nanobioscience, Yokohama City University, Yokohama, Japan.
International College of Arts and Sciences, Yokohama City University, Yokohama, Japan.
In Vivo. 2019 Jul-Aug;33(4):1095-1102. doi: 10.21873/invivo.11578.
BACKGROUND/AIM: Perinatal diethylstilbestrol (DES) treatment induces the polyovular follicle containing two or more oocytes in a follicle of mouse ovary through estrogen receptor (ER) β. The aim of the study was to investigate the direct effects of DES on the neonatal mouse ovary and the gene expression of activins.
Ovaries from neonatal wild-type (WT) or ERβ- knockout (ERβKO) mice were organ-cultured in a serum-free medium with or without DES, and polyovular follicle induction and expression of activin signaling related genes were examined.
The polyovular follicle and cyst incidence in DES-treated organ-cultured ovaries from WT mice, but not from ERβKO mice, was significantly higher than that of control non-treated cultures. DES altered inhibin (Inh) a, Inhba and Inhbb expression in organ-cultured ovaries from C57BL/6J mice, while no change in Inha and an increase of Inhbb were observed by DES, in both WT and ERβKO mice.
Alterations in activin signaling are involved in the polyovular follicle induction by DES.
背景/目的：围产期己烯雌酚（DES）通过雌激素受体（ER）β诱导小鼠卵巢卵泡中含有两个或更多卵母细胞的多卵泡。本研究的目的是研究 DES 对新生小鼠卵巢的直接影响及其激活素基因的表达。
将来自新生野生型（WT）或 ERβ-敲除（ERβKO）小鼠的卵巢在无血清培养基中进行器官培养，有或没有 DES，并检查多卵泡诱导和激活素信号相关基因的表达。
与对照未处理的培养物相比，DES 处理的 WT 小鼠卵巢器官培养物中的多卵泡和囊泡发生率明显更高，但在 ERβKO 小鼠中则没有。DES 改变了 C57BL/6J 小鼠卵巢器官培养物中抑制素（Inh）a、Inhba 和 Inhbb 的表达，而在 WT 和 ERβKO 小鼠中，DES 观察到 Inha 没有变化，Inhbb 增加。
激活素信号的改变参与了 DES 诱导的多卵泡发生。
