Grünenthal GmbH, Grünenthal Innovation, Aachen, Germany.
Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
In Vivo. 2019 Jul-Aug;33(4):1125-1132. doi: 10.21873/invivo.11582.
Cancer-induced bone pain remains a serious public health concern, with a need for translational behavioural tests in order to assess nociception in preclinical models of this condition. Burrowing is an innate, ethologically relevant rodent behaviour that has been proven sensitive to chronic pain conditions. Herein, we studied for the first time whether burrowing performance is altered in preclinical models of cancer-induced bone pain.
Mice and rats were inoculated with syngeneic breast cancer cells. Bone degradation was radiographically evaluated and nociception was assessed in limb-use and burrowing tests.
Cancer-bearing rodents showed reduced relative bone density and limb-use scores, confirming disease development. Burrowing performance decreased over time in both rodent models.
Burrowing performance was reduced in both rodent models, indicating that the burrowing test is a relevant and reproducible behavioural test for assessing disease development in both mouse and rat models of cancer-induced bone pain.
癌症引起的骨痛仍然是一个严重的公共卫生问题，需要转化行为测试，以便在这种疾病的临床前模型中评估伤害感受。打洞是一种先天的、与行为相关的啮齿动物行为，已被证明对慢性疼痛状况敏感。在此，我们首次研究了打洞表现是否会在癌症引起的骨痛的临床前模型中发生改变。
将同源乳腺癌细胞接种到小鼠和大鼠体内。通过放射照相评估骨降解，并在肢体使用和打洞测试中评估伤害感受。
患有癌症的啮齿动物表现出相对骨密度和肢体使用评分降低，证实了疾病的发展。在两种啮齿动物模型中，打洞表现随时间推移而降低。
两种啮齿动物模型的打洞表现均降低，表明打洞测试是一种相关且可重复的行为测试，可用于评估小鼠和大鼠癌症引起的骨痛模型中的疾病发展。
