高卡路里饮食诱导的胰腺损伤中 Podoplanin 和 PROX1 的表达。
Podoplanin and PROX1 Expression in Hypercaloric Diet-induced Pancreatic Injuries.
机构信息
Department of Microscopic Morphology/Histology, Victor Babes University of Medicine and Pharmacy, Timisoara, Romania.
Angiogenesis Research Center, Victor Babes University of Medicine and Pharmacy, Timisoara, Romania.
出版信息
In Vivo. 2019 Jul-Aug;33(4):1157-1163. doi: 10.21873/invivo.11586.
BACKGROUND
The role of podoplanin (PDPN) and homebox prospero gene 1 (PROX1) in early stages of pancreatic islet changes induced by hypercaloric diet is unclear. The aim of this study was to study PDPN and PROX1 variability in pancreatic islets after a hypercaloric diet in a rat experimental model.
MATERIALS AND METHODS
Pancreatic biopsies harvested from Sprague-Dawley rats at 3, 6, and 9 weeks following hypercaloric diet intake were evaluated for morphological and molecular changes of Langerhans islets based on PDPN and PROX1 expression Results: Six weeks of hypercaloric diet induced hypertrophy of pancreatic islets with focal expression of Pdpn and Prox1 mRNA. At 9 weeks of hypercaloric diet, strong peri-insular inflammation was found around hypertrophic islets highly expressing PDPN, and lacking Prox1 mRNA and protein expression.
CONCLUSION
This is the first report of Pdpn and Prox1 mRNA expression variability and involvement in early steps of pancreatic islet changes following hypercaloric food intake.
背景
在高热量饮食诱导的胰岛早期变化中，足细胞蛋白（PDPN）和同源框prospero 基因 1（PROX1）的作用尚不清楚。本研究旨在研究高热量饮食诱导的大鼠实验模型中胰岛内 PDPN 和 PROX1 的变异性。
材料和方法
从高热量饮食摄入后 3、6 和 9 周的 Sprague-Dawley 大鼠的胰腺活检中，根据 PDPN 和 PROX1 表达评估胰岛的形态和分子变化。结果：6 周的高热量饮食导致胰岛肥大，Pdpn 和 Prox1 mRNA 呈局灶性表达。在高热量饮食 9 周时，在高表达 PDPN 的肥大胰岛周围发现了强烈的胰岛周围炎症，缺乏 Prox1 mRNA 和蛋白表达。
结论
这是高热量食物摄入后胰岛变化早期 Pdpn 和 Prox1 mRNA 表达变异性及其参与的首次报道。
Zotero 插件