Division of Gastroenterology and Hepatology, Department of Internal Medicine, Toho University Graduate School of Medicine, Ota-ku, Tokyo, Japan.
Division of Gastroenterology and Hepatology, Department of Internal Medicine, Toho University Medical Center Omori Hospital, Ota-ku, Tokyo, Japan.
Histol Histopathol. 2022 Jun;37(6):587-595. doi: 10.14670/HH-18-445. Epub 2022 Feb 28.
To elucidate tumor cell behavior associated with cancer stem cell (CSC) marker expression, the expression of CD133, CD44v9, and ALDH1A1, which are considered markers of CSCs, was examined in sporadic and ulcerative colitis (UC)-associated colorectal tumors.
A total of 23 cases of sporadic colorectal cancer and 44 cases of adenoma were collected. Additionally, 22 cancer lesions and 38 dysplasia lesions were selected from 28 colectomy cases of UC with neoplastic lesions. Lesions were examined by immunohistochemistry using primary antibodies against CD133, CD44v9, ALDH1A1, Ki-67, cleaved-Caspase 3, and p53.
CD133, CD44v9, and ALDH1A1 showed higher expression in both sporadic and UC-associated tumors than in the normal mucosa. ALDH1A1 expression in sporadic cancer was higher in the right colon than in the left colon (p=0.0089). ALDH1A1 expression in UC-associated cancer was higher in those with longer disease duration than in those with shorter disease duration (p=0.019). The CD44v9+/CD133- region had fewer cleaved-Caspase 3 positive cells in both sporadic and UC-associated cancers. In sporadic cancer, CD133+/ALDH1A1+ regions had fewer apoptotic cells than CD133+/ALDH1A1- regions, while CD133+/ALDH1A1- regions were less proliferative than CD133+/ALDH1A1+ regions in UC-associated cancer.
CD44+/CD133- regions were commonly associated with low apoptosis in sporadic and UC-associated cancers; thus, these were considered target areas for CSCs. Additionally, the combination of markers comprising CSCs may differ between sporadic and UC-associated cancers.
为了阐明与癌症干细胞（CSC）标志物表达相关的肿瘤细胞行为，我们检测了散发性和溃疡性结肠炎（UC）相关结直肠肿瘤中 CD133、CD44v9 和 ALDH1A1 的表达，这些标志物被认为是 CSC 的标志物。
共收集了 23 例散发性结直肠癌和 44 例腺瘤病例。此外，从 28 例有肿瘤病变的 UC 结肠切除术病例中选择了 22 个癌病变和 38 个异型增生病变。使用针对 CD133、CD44v9、ALDH1A1、Ki-67、cleaved-Caspase 3 和 p53 的一抗，通过免疫组织化学方法检查病变。
CD133、CD44v9 和 ALDH1A1 在散发性和 UC 相关肿瘤中的表达均高于正常黏膜。散发性结肠癌中右半结肠的 ALDH1A1 表达高于左半结肠（p=0.0089）。UC 相关癌症中，疾病持续时间较长的患者的 ALDH1A1 表达高于疾病持续时间较短的患者（p=0.019）。在散发性和 UC 相关癌症中，CD44v9+/CD133-区域的 cleaved-Caspase 3 阳性细胞较少。在散发性癌症中，CD133+/ALDH1A1+区域的凋亡细胞少于 CD133+/ALDH1A1-区域，而在 UC 相关癌症中，CD133+/ALDH1A1-区域的增殖细胞少于 CD133+/ALDH1A1+区域。
CD44+/CD133-区域通常与散发性和 UC 相关癌症中的低凋亡相关；因此，这些区域被认为是 CSC 的靶区。此外，CSC 标志物的组合在散发性和 UC 相关癌症中可能不同。
