Chouchkov C, Lazarov N, Ichev K
Department of Anatomy, Higher Medical Institute in Stara Zagora, Bulgaria.
Acta Histochem. 1987;82(2):153-8. doi: 10.1016/S0065-1281(87)80021-1.
Using light- and electron microscope radioautography, the dynamics of newly synthesized protein precursors taking part in elaboration of brain basement membrane have been examined. The data presented give evidence that all cell types (endothelial, pericytal, and astroglial cells) surrounding the brain basement membrane contribute to its composition. A quantitative uptake analysis of 3H-proline indicates a progressive decline in the amount of labeled precursor in the examined cell types with a corresponding increase in deposition of the label at the presumptive basement membrane. The endothelial cells plays the main role in the membrane elaboration followed by pericyte and astrocyte which participate at lesser but equal degree in this synthesis.
利用光镜和电镜放射自显影技术，对参与脑基底膜形成的新合成蛋白质前体的动态变化进行了研究。所提供的数据表明，围绕脑基底膜的所有细胞类型（内皮细胞、周细胞和星形胶质细胞）都对其组成有贡献。对³H-脯氨酸的定量摄取分析表明，在所研究的细胞类型中，标记前体的量逐渐下降，而在假定的基底膜处标记物的沉积相应增加。内皮细胞在膜的形成中起主要作用，其次是周细胞和星形胶质细胞，它们在这种合成中参与程度较小但相同。
