Department of Microbiology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.
J Environ Sci Health B. 2019;54(10):832-842. doi: 10.1080/03601234.2019.1636601. Epub 2019 Jul 7.
This study was aimed to evaluate the effect of a mixture of flufenacet + isoxaflutole on counts of microorganisms, ecophysiological diversity index (EP), colony development index (CD) and on the enzymatic activity of soil and maize growth. The experiment was conducted with sandy clay, to which the tested herbicide was administered in doses of: 0.25, 5.0, 10, 20, 40, 80 and 160 mg/kg. Soil without the addition of the mixture served as the control. Results demonstrated that the tested mixture contributed to a decrease in numbers of , organotrophic bacteria, actinobacteria and fungi. The negative effect of the herbicide could also be noticed in the case of the enzymatic activity of soil. Soil contamination contributed to suppressed activities of dehydrogenases, catalase, urease, alkaline phosphatase and arylsulfatase. In turn, the initial increase in the activity of β-glucosidase was followed by its decline observed with time. The flufenacet + isoxaflutole mixture affected also maize plant growth, reducing maize dry matter yield when used at doses from 5.0 to 160 mg/kg. In summary, it may be concluded that mixture evokes a negative effect on the microbiological and biochemical activity of soil and that their excess in the soil leads to plant decay as at the seeding stage.
本研究旨在评估氟噻草胺+异恶草松混合物对微生物数量、生态生理多样性指数（EP）、菌落发育指数（CD）以及土壤酶活性和玉米生长的影响。该实验在砂壤土上进行，向其中施用以 0.25、5.0、10、20、40、80 和 160mg/kg 的剂量的测试除草剂。未添加混合物的土壤作为对照。结果表明，测试混合物导致了数量减少，有机营养细菌、放线菌和真菌。除草剂的负面影响也可以在土壤的酶活性中注意到。土壤污染导致脱氢酶、过氧化氢酶、脲酶、碱性磷酸酶和芳基硫酸酯酶的活性受到抑制。相反，β-葡萄糖苷酶的初始活性增加，随后随着时间的推移观察到其活性下降。氟噻草胺+异恶草松混合物还影响玉米植物的生长，当剂量为 5.0 至 160mg/kg 时，降低了玉米干物质产量。总之，可以得出结论，混合物对土壤的微生物和生化活性产生负面影响，并且它们在土壤中的过量会导致植物在播种阶段腐烂。
