Association of mutations and genetic polymorphisms in lung adenocarcinoma patients.

High-mobility group protein box 1 (HMGB1) is overexpressed and reported to be a prognostic factor in patients with non-small-cell lung cancer (NSCLC). Epidermal growth factor receptor (EGFR) mutants play an important role in NSCLC progression. The aim of this study was to explore potential associations between genetic polymorphisms of and mutations in a cohort that included 280 patients with NSCLC, some of whom were smokers and others who never smoked. Four tagged single-nucleotide polymorphisms (SNPs) of were detected by a TaqMan-based real-time polymerase chain reaction (PCR) in patients. We found that after adjusting for other covariates, NSCLC patients who smoked and who respectively had CG, CT, and TC heterozygotes of rs2249825, rs1045411, and rs1360485, were at lower risk of developing mutant , compared to those patients with wild-type homozygotes. Moreover, significant inverse associations between the CG and CG + GG genotypes of rs2249825 and the hotspot mutation, an exon 19 in-frame deletion, were also observed among NSCLC patients. Within patients harboring mutant , rs1360485 C (TC + CC) allele carriers were at higher risk of developing poorly differentiated cancer types (odds ratio=5.493, 95% confidence interval: 1.130~26.696, =0.019), compared to patients with TT homozygotes. Furthermore, we found that rs1360485 polymorphisms seemed to be related to susceptibility to developing poorly differentiated cancer linked to tobacco consumption in mutant patients. In conclusion, our results suggested that variants are significantly inversely associated with mutations among NSCLC patients who smoked. variants and tobacco consumption might contribute to the pathological development of NSCLC.