三酰甘油脂肪酶介导花生四烯酸的释放以用于肾髓质前列腺素的合成。

Triacylglycerol lipase-mediated release of arachidonic acid for renal medullary prostaglandin synthesis.

作者信息

Fujimoto Y, Nishioka K, Hase Y, Sado K, Fujita T

机构信息

Department of Hygienic Chemistry, Osaka University of Pharmaceutical Sciences, Japan.

出版信息

Arch Biochem Biophys. 1988 Mar;261(2):368-74. doi: 10.1016/0003-9861(88)90352-9.

DOI:10.1016/0003-9861(88)90352-9

PMID:3128171

Abstract

The effect of triacylglycerol lipase or triarachidonin on the synthesis of prostaglandin E2 in rabbit kidney medulla slices was examined. Prostaglandin E2 generation was enhanced by exogenous triacylglycerol lipase, indicating that arachidonic acid released from medullary triacylglycerols is readily available for prostaglandin biosynthesis. Triarachidonin stimulated prostaglandin E2 production in a dose-dependent manner. Moreover, the kidney medulla slices showed triacylglycerol lipase activity using trimargarin as substrate. The addition of mepacrine made the stimulatory effect of triarachidonin on prostaglandin E2 formation more pronounced. Studies utilizing EGTA and p-bromophenacyl bromide revealed that triacylglycerol lipase of kidney medulla is independent of Ca2+ and sensitive to a sulfhydryl inhibitor. These results suggest the presence of triacylglycerol lipase and triacylglycerol as a possible candidate for providing free arachidonic acid to cyclooxygenase in kidney medulla.

摘要

研究了三酰甘油脂肪酶或三花生四烯酸甘油酯对兔肾髓质切片中前列腺素E2合成的影响。外源性三酰甘油脂肪酶可增强前列腺素E2的生成，这表明从髓质三酰甘油释放的花生四烯酸易于用于前列腺素生物合成。三花生四烯酸甘油酯以剂量依赖性方式刺激前列腺素E2的产生。此外，肾髓质切片以三软脂酸甘油酯为底物表现出三酰甘油脂肪酶活性。加入米帕林使三花生四烯酸甘油酯对前列腺素E2形成的刺激作用更加明显。利用乙二醇双四乙酸（EGTA）和对溴苯甲酰溴进行的研究表明，肾髓质的三酰甘油脂肪酶不依赖于Ca2+，且对巯基抑制剂敏感。这些结果表明肾髓质中存在三酰甘油脂肪酶和三酰甘油，它们可能是为环氧化酶提供游离花生四烯酸的候选物质。