Gruhn K, Zander R, Kirchner E
Karl-Marx-Universität Leipzig, Wissenschaftsbereich Tierernährungschemie.
Arch Tierernahr. 1987 Sep;37(9):765-75. doi: 10.1080/17450398709428246.
12 colostomized laying hens which received 15N labelled wheat over 4 days were butchered 12 h, 36 h, 60 h and 108 h (3 animals each) after the last 15N application. The intake of 15N excess (15N') from the wheat amounted to 540 mg 15N' during the application period. The 15N' in the blood plasma decreased after the last 15N' application from 0.76 atom-% to 0.55 atom-% after 108 h, the labelling of the corpuscular components at the same measuring points increased from 0.28 to 0.50 atom-% 15N'. 96.6% of the plasma 15N' and 93.8% of that in the corpuscles is precipitable in trichloric acetic acid. The atom-% 15N' of histidine in the total blood remained unchanged in dependence on the butchering time. The 15N amount in lysine and arginine and that in the non-basic amino acids decreased inconsiderably in the period between 12 h and 108 h after the last 15N' wheat feeding.
12只接受了4天15N标记小麦的结肠造口产蛋母鸡，在最后一次施用15N后12小时、36小时、60小时和108小时（每组3只）被宰杀。在施用期间，从小麦中摄入的过量15N（15N'）量达到540毫克15N'。最后一次施用15N'后，血浆中的15N'在108小时后从0.76原子%降至0.55原子%，在相同测量点，细胞成分的标记从0.28原子%增加到0.50原子% 15N'。血浆中96.6%的15N'和细胞中93.8%的15N'可在三氯乙酸中沉淀。全血中组氨酸的15N'原子%不依赖于宰杀时间保持不变。在最后一次喂食15N标记小麦后的12小时至108小时期间，赖氨酸和精氨酸中的15N量以及非碱性氨基酸中的15N量略有下降。
